Evidence map›Paper›PMID 42818391›Full record

ArticlebioRxiv : the preprint server for biology2026

Three dimensional reconstruction of the human nucleus accumbens reveals topographic organization and molecular heterogeneity of D1-islands across the anterior posterior axis.

Robert A Phillips, Jianing Yao, Svitlana V Bach, Ishbel Del Rosario Alvia, Yufeng Du, Sarah E Maguire, Ruth Zhang, Ryan A Miller, Joel E Kleinman, Thomas M Hyde and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Robert A PhillipsLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0003-3560-4747
Jianing YaoDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.ORCID 0009-0004-8213-2118
Svitlana V BachLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0003-1134-9585
Ishbel Del Rosario AlviaLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0001-6225-0660
Yufeng DuLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0009-0002-3915-5205
Sarah E MaguireLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0009-0005-2884-4787
Ruth ZhangLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0009-0003-2134-0952
Ryan A MillerLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0003-3477-7443
Joel E KleinmanLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0002-4210-6052
Thomas M HydeLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0002-8746-3037
Keri MartinowichLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0002-5237-0789
Kristen R MaynardLieber Institute for Brain Development, Johns Hopkins Medical Campus, Baltimore, MD, USA.ORCID 0000-0003-0031-8468
Stephanie C HicksDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.ORCID 0000-0002-7858-0231

Funding

Cellular resolution spatio-molecular expression across the anterior-posterior axis of the nucleus accumbens in the human brainR01DA065276 · NIDA · LIEBER INSTITUTE, INC. · PI Stephanie Carinne Hicks, Keri Martinowich · 2026 to 2026
$1.9M
Identifying a functional role for transcriptionally distinct, BLA-activated LS ensembles in social behaviorsF32MH139150 · NIMH · LIEBER INSTITUTE, INC. · PI Robert Phillips · 2024 to 2026
$161k
NIDA NIH HHS R01 DA065276NIMH NIH HHS F32 MH139150
6 · The paper itself

Abstract

Spatial transcriptomics has transformed molecular characterization of the human brain, but most studies profile individual two-dimensional tissue sections and therefore cannot capture molecular organization across 3D neuroanatomical axes. Here, we developed a spatial genomics framework to reconstruct the 3D cellular architecture of the human nucleus accumbens (NAc) by densely sampling and aligning serial sections across its anterior-posterior (AP) extent. Combining single-cell Xenium profiling with near-transcriptome-wide VisiumHD, we mapped cell type composition and topography across AP, mediolateral and dorsoventral axes. Neuronal populations varied in relative abundance across the AP axis while maintaining characteristic spatial topographies. These differences were particularly pronounced among D1-islands, a topographically organized structure within the NAc in which transcriptionally distinct subpopulations of

Indexed as

3D reconstructionD1-islandsdopaminenucleus accumbensSpatial transcriptomics

Identifiers

PMID42818391
PMCPMC13622273

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.