Evidence map›Paper›PMID 42818105›Full record

ArticlebioRxiv : the preprint server for biology2026

Folate deficiency disrupts key metabolic transitions within the developing neural ectoderm.

Nicolas Dias, Daniel P Lewinsohn, William N Colgan, Minming Wang, Yusuke Kijima, JoAnne Villagrana, Tien-Chi Jason Hou, Gokul Gowri, Adelina Chau, Tuğçe Aktaş and 5 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Nicolas DiasDepartment of Genetics and the Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, CT, USA.ORCID 0000-0003-0873-7245
Daniel P LewinsohnCenter for Computational Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID 0000-0002-4868-7847
William N ColganWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0002-8328-4672
Minming WangDepartment of Genetics and the Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, CT, USA.ORCID 0000-0002-5377-5518
Yusuke KijimaMax Planck Institute for Molecular Genetics, Berlin, Germany.ORCID 0000-0002-7934-6762
JoAnne VillagranaDepartment of Genetics and the Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, CT, USA.
Tien-Chi Jason HouDepartment of Genetics and the Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, CT, USA.ORCID 0000-0002-8349-3132
Gokul GowriWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0003-0911-6889
Adelina ChauDepartment of Electrical Engineering and Computer Sciences, University of California, Berkeley, Berkeley, CA, USA.ORCID 0009-0002-1691-8732
Tuğçe AktaşMax Planck Institute for Molecular Genetics, Berlin, Germany.ORCID 0000-0003-1599-9454
Kaelyn SumigrayDepartment of Genetics and the Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, CT, USA.ORCID 0000-0002-1267-7559
Jonathan S WeissmanWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0003-2445-670X
Luke W KoblanWhitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.ORCID 0000-0003-2814-4412
Allon WagnerCenter for Computational Biology, University of California, Berkeley, Berkeley, CA, USA.ORCID 0000-0001-5095-6009
Zachary D SmithDepartment of Genetics and the Yale Stem Cell Center, Yale School of Medicine, Yale University, New Haven, CT, USA.ORCID 0000-0002-9283-1957

Funding

Center for Genomic Editing and Recording: Development and Application of Next-Generation Genome and Epigenome Editing Methods to Advance the Study and Treatment of Human DiseaseRM1HG009490 · NHGRI · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI Brittany S. Adamson, Martin Joseph Ankrah Aryee · 2017 to 2026
$22.7M
Epigenetic and environmental impact on early embryonic developmentDP2HD108774 · NICHD · YALE UNIVERSITY · PI SMITH, ZACHARY DYLAN · 2021 to 2024
$2.5M
Spatially-resolved, integrated cell state and lineage tracing to define progenitor cell dynamics in early mouse developmentK99HD118574 · NICHD · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI Luke William Koblan · 2025 to 2026
$250k
NHGRI NIH HHS RM1 HG009490NICHD NIH HHS DP2 HD108774NICHD NIH HHS K99 HD118574
6 · The paper itself

Abstract

Despite long-standing epidemiological associations, the mechanism linking folate availability to gestational neural tube defects remains unclear, partly because measuring and interpreting metabolic activity in dynamic biological systems remains challenging. Here, we apply a deep-learning-based graph-guided variational autoencoder (MeRN; Metabolic Representation Network) to infer single-cell metabolic activity and states from scRNA-seq data of mouse embryogenesis. By analyzing folate-deficient embryogenesis from E7.0-E9.0, we identify a transient state within the nascent neural lineage that is acutely sensitive to folate availability, leading to an interconnected disruption between key bioenergetic pathways and

Indexed as

Congenital DisordersDeep learningEmbryonic DevelopmentFolate MetabolismMorphogenesisNeurogenesisSingle-cell transcriptomicsVariational Autoencoders

Identifiers

PMID42818105
PMCPMC13622060

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.