Evidence map›Paper›PMID 42818034›Full record

ArticlebioRxiv : the preprint server for biology2026

Structural and genetic dissection of RNA-guided gene repression by TnpB-derived transcription factors.

Renjian Xiao, Americo A Casas-Ciniglio, Hoang C Le, Tanner Wiegand, Dan Xie, Leifu Chang, Samuel H Sternberg

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Renjian XiaoDepartment of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907, USA.ORCID 0000-0002-5914-0812
Americo A Casas-CiniglioDepartment of Genetics and Development, Columbia University, New York, NY, USA.ORCID 0009-0001-9785-9860
Hoang C LeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0009-0003-7911-881X
Tanner WiegandDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0000-0002-0528-268X
Dan XieDepartment of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907, USA.
Leifu ChangDepartment of Biological Sciences, Purdue University, 915 W. State Street, West Lafayette, IN 47907, USA.ORCID 0000-0002-6107-5271
Samuel H SternbergDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0000-0001-8240-9114

Funding

Molecular basis for CRISPR RNA-guided nucleic acid cleavage and DNA transpositionR01GM138675 · NIGMS · PURDUE UNIVERSITY · PI CHANG, LEIFU · 2020 to 2024
$1.8M
Molecular basis for CRISPR-associated transposons and RNA-guided transcriptional regulationR35GM158248 · NIGMS · PURDUE UNIVERSITY · PI Leifu Chang · 2025 to 2026
$923k
NIGMS NIH HHS R01 GM138675NIGMS NIH HHS R35 GM158248
6 · The paper itself

Abstract

TnpB nucleases, the evolutionary progenitors of CRISPR-associated Cas12 enzymes, are transposon-encoded, RNA-guided endonucleases found throughout bacteria. Independent of the trajectory towards adaptive immunity, TnpB nucleases have also recurrently given rise to TnpB-like nuclease-dead repressors (TldRs), a family of programmable RNA-guided transcription factors, though the physiological roles of most TldR clades remain unclear. Recently, we identified a TldR clade associated with bacterial ABC transporter operons, but this clade has not been characterized in any native host, leaving the biological significance of its predicted regulatory function unknown. Here, we demonstrate direct

Identifiers

PMID42818034
PMCPMC13622181

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.