ArticleSensors (Basel, Switzerland)2026
Quantitative Determination of Prostate-Specific Antigen in Forensic Samples Using Antibody Microarrays on 3D Plasma Nanotextured Polymeric Slides.
Article in Sensors (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Semen identification in forensic samples using prostate-specific antigen (PSA) overcomes limitations of conventional methods such as UV illumination and spermatozoa detection. In this study, we describe an antibody microarray for the quantitative immunochemical detection of PSA in forensic samples using 3D oxygen plasma-nanotextured polymer-coated glass slides. The analytical performance of these substrates was compared with that of silane-modified glass slides and demonstrated improved intra- and inter-spot homogeneity, as well as an extended linear dynamic range. Based on the 3D nanotextured slides, a two-step non-competitive immunoassay was developed using a biotinylated anti-PSA antibody and Alexa Fluor 546-labeled streptavidin for fluorescence detection. The assay was completed within 1 h, achieved a detection limit of 0.05 ng/mL PSA, and enabled semen detection at dilutions of up to 2 × 10
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