ArticleReproduction in domestic animals = Zuchthygiene2026
Effects of Sucrose and Trehalose Supplementation on Zebrafish (Danio rerio) Sperm Quality During Cold Storage.
Article in Reproduction in domestic animals = Zuchthygiene, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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9 authors.
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Abstract
This study evaluated the effect of disaccharide supplementation on zebrafish sperm stored at 5°C for 24 h. Seven pooled replicates from 60 broodfish were used. Semen samples were initially cooled and, after 1 h, supplemented with either HBSS (E-C), Sucrose 0.13M (E-S13), Trehalose 0.13M (E-T13) or Trehalose 0.26M (E-T26). Sperm motility (computer-assisted sperm analysis) and plasma membrane integrity (flow cytometry) were evaluated over 24 h of cool storage. During the first 2 h, E-T26 decreased sperm motility values. After 24 h, E-S13 and E-T13 showed higher motility than E-C and E-T26. In conclusion, sucrose and trehalose supplementation improved sperm motility after 24 h of cold storage. Further studies are needed to assess the effect of disaccharide supplementation after shorter storage periods.
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