Evidence map›Paper›PMID 42814837›Full record

ArticleScience advances2026

Toward transgenic multicellular parasites: CRISPR-Cas12a germline editing of

Max F Moescheid, Prapakorn Wisitpongpun, Vanessa Gross, Victoria H Mann, Thomas Quack, Anne Rognon, Christoph Grunau, Wannaporn Ittiprasert, Paul J Brindley, Christoph G Grevelding

Abstract read
In one paragraph

Article in Science advances, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Max F MoescheidInstitute of Parasitology, Biomedical Research Center Seltersberg (BFS), Justus Liebig University Giessen, Germany.ORCID 0000-0001-5007-3687
Prapakorn WisitpongpunDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.ORCID 0009-0006-2624-0047
Vanessa GrossInstitute of Parasitology, Biomedical Research Center Seltersberg (BFS), Justus Liebig University Giessen, Germany.ORCID 0009-0008-9908-1352
Victoria H MannDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.
Thomas QuackInstitute of Parasitology, Biomedical Research Center Seltersberg (BFS), Justus Liebig University Giessen, Germany.ORCID 0000-0001-7079-5206
Anne RognonIHPE Univ Perpignan Via Domitia, CNRS, IFREMER, Univ Montpellier, Perpignan, France.ORCID 0000-0002-4197-8879
Christoph GrunauIHPE Univ Perpignan Via Domitia, CNRS, IFREMER, Univ Montpellier, Perpignan, France.ORCID 0000-0002-2161-412X
Wannaporn IttiprasertDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.ORCID 0000-0001-9411-8883
Paul J BrindleyDepartment of Microbiology, Immunology & Tropical Medicine, School of Medicine & Health Sciences, George Washington University, Washington, DC 20037, USA.ORCID 0000-0003-1765-0002
Christoph G GreveldingInstitute of Parasitology, Biomedical Research Center Seltersberg (BFS), Justus Liebig University Giessen, Germany.ORCID 0000-0002-3144-2026

Funding

Targeting parasite-host communication to combat liver fluke-induced bile duct cancerR01CA164719 · NCI · GEORGE WASHINGTON UNIVERSITY · PI Paul J Brindley, THEWARACH LAHA · 2012 to 2026
$5.0M
Acquisition of Zeiss 710 Confocal Laser Scanning MicroscopeS10RR025565 · NCRR · GEORGE WASHINGTON UNIVERSITY · PI POPRATILOFF, ANASTAS · 2009 to 2009
$500k
NCI NIH HHS R01 CA164719NCRR NIH HHS S10 RR025565NIAID NIH HHS HHSN272201700014CWellcome Trust
6 · The paper itself

Abstract

Lack of transgenesis methods impedes functional studies in platyhelminths. We present a substantially improved ribonucleic protein complex (RNP)-based genome editing approach for

Indexed as

CRISPR-Cas SystemsGene EditingSchistosoma mansoniAnimalsAnimals, Genetically ModifiedCricetinaeGerm CellsGreen Fluorescent ProteinsOvumGreen Fluorescent Proteins

Identifiers

PMID42814837
PMCPMC13626086

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.