Evidence map›Paper›PMID 42814364›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Single-Cell RNA-Sequencing in Nodulated Roots of Medicago truncatula.

Sophia Müller, Kelvin Adema, Amber van Seters, Jana Witmer, Wouter Kohlen, Carolin Grones

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sophia MüllerDepartment of Plant Sciences, Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands.ORCID http://orcid.org/0009-0005-8755-4202
Kelvin AdemaDepartment of Plant Sciences, Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands.ORCID http://orcid.org/0009-0005-6892-1345
Amber van SetersDepartment of Plant Sciences, Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands.ORCID http://orcid.org/0009-0009-3772-8189
Jana WitmerDepartment of Plant Sciences, Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands.ORCID http://orcid.org/0009-0004-6575-4724
Wouter KohlenDepartment of Plant Sciences, Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands. wouter.kohlen@wur.nl.ORCID https://orcid.org/0000-0001-9057-2392
Carolin GronesDepartment of Plant Sciences, Cluster of Plant Developmental Biology, Laboratory of Cell and Developmental Biology, Wageningen University, Droevendaalsesteeg 1, Wageningen, 6708 PB, The Netherlands. carolin.grones@wur.nl.ORCID https://orcid.org/0000-0002-8962-3778

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This section details the optimized methods for single-cell RNA sequencing (scRNA-seq) in Medicago truncatula roots and nodules. We describe a detailed experimental workflow, comprising plant growth and bacteria application, protoplast isolation, quality control, and library processing according to 10× Genomics

Indexed as

Medicago truncatulaPlant Root NodulationPlant RootsRoot Nodules, PlantSequence Analysis, RNASingle-Cell AnalysisGene Expression Regulation, PlantHigh-Throughput Nucleotide SequencingProtoplastsRNA, PlantSingle-Cell Gene Expression AnalysisRNA, PlantMedicago truncatulaNoduleProtoplastSingle-cell RNA sequencing

Identifiers

PMID42814364

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.