Evidence map›Paper›PMID 42814336›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Build Heatmaps of Cell Frequencies and Marker Intensities for Correct Interpretations.

Eugénie Lohmann, Laurent Gorvel, Samuel Granjeaud

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Eugénie LohmannCentre de Recherche en Cancérologie de Marseille, CRCM, Institut Paoli-Calmettes, Inserm, CNRS, Aix Marseille University, Marseille, France.ORCID http://orcid.org/0000-0002-3230-8363
Laurent GorvelCentre de Recherche en Cancérologie de Marseille, CRCM, Institut Paoli-Calmettes, Inserm, CNRS, Aix Marseille University, Marseille, France.ORCID http://orcid.org/0000-0001-7526-261X
Samuel GranjeaudCentre de Recherche en Cancérologie de Marseille, CRCM, Institut Paoli-Calmettes, Inserm, CNRS, Aix Marseille University, Marseille, France. samuel.granjeaud@inserm.fr.ORCID http://orcid.org/0000-0001-9245-1535

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

High-content cytometry is an important technique in clinical research, producing data that is rich and complex to analyze. The resulting clusters of cells must be profiled to determine their role and function and analyzed to identify changes in cell frequency or marker intensity. The heat map is the most suitable tool for presenting this information in a synthetic way. However, its construction must be adapted to the information and the objective. Here we describe the step-by-step construction of heat maps of marker intensity and cell frequency using Excel, Phantasus, or R, including the conversion of numbers to colors and the organization of rows and columns. Researchers who master these steps will be able to correctly interpret heat maps and derive the most benefit from high-content cytometry.

Indexed as

BiomarkersFlow CytometryCell CountHumansSoftwareBiomarkersBuildCytometryFrequencyHeat mapHeatmapIntensityMethodPhantasusPheatmap

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.