Evidence map›Paper›PMID 42814255›Full record

ArticleMikrochimica acta2026

Phosphorylation-asymmetric recombinase polymerase amplification enables target-directed circularization for ultrasensitive multiplex electrochemical detection of respiratory pathogens.

Yemin Han, Haotian Yu, Manman Lv, Mengqing Cheng, Houyang Ge, Song Gao, Lu Yin, Qinyu Ge, Xinping Xu, Yan Huang and 3 more

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yemin HanState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Haotian YuState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Manman LvState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Mengqing ChengState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Houyang GeState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Song GaoState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Lu YinJiangxi Provincial Key Laboratory of Respiratory Diseases, The Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, China.
Qinyu GeState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Xinping XuJiangxi Provincial Key Laboratory of Respiratory Diseases, The Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, China.
Yan HuangState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Longhua SunJiangxi Provincial Key Laboratory of Respiratory Diseases, The Department of Respiratory and Critical Care Medicine, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, 330052, China. ndyfy05715@ncu.edu.cn.
Zuhong LuState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China.
Quanjun LiuState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, No. 2, Jiulonghu, Nanjing, 211189, People's Republic of China. lqj@seu.edu.cn.

Funding

The First Affiliated Hospital of Nanchang University Clinical Research Seed Program No. YFYLCYJPY202516the National Natural Science Foundation of China 61827814, 31872726the Shenzhen Fundamental Research Program No. JCYJ20230807114612024
6 · The paper itself

Abstract

Target-directed circularization has emerged as an efficient strategy for rolling circle amplification (RCA); however, its application to double-stranded DNA (dsDNA) remains challenging because genomic DNA cannot directly generate ligation-ready circular templates. Herein, we report a phosphorylation-asymmetric recombinase polymerase amplification (PA-RPA) strategy that directly converts dsDNA into ligation-ready single-stranded templates, thereby enabling target-directed circularization without requiring predesigned padlock probes. To facilitate multiplex analysis, a universal terminal sequence was incorporated into all PA-RPA products, allowing different targets to share a common bridge oligonucleotide for circular template construction and substantially simplifying assay design. The generated circular templates initiate Phi29 polymerase-mediated RCA, while Cas12a-mediated cleavage releases secondary primers that continuously regenerate amplification intermediates through a positive-feedback circuit. Coupled with a signal-off electrochemical transduction platform based on stem-loop probes immobilized on screen-printed carbon electrodes, the proposed strategy enabled simultaneous detection of Staphylococcus aureus (S. aureus) and Acinetobacter baumannii (A. baumannii). The biosensor achieved detection limits of 0.33 aM and 0.45 aM for S. aureus and A. baumannii, respectively, with a linear range from 1 aM to 10 pM, together with excellent specificity, satisfactory storage stability, and reliable analytical performance in simulated clinical samples. By establishing a general template-conversion strategy for target-directed circularization of dsDNA and a universal circularization design compatible with multiplex analysis, this work expands the applicability of RCA-based molecular diagnostics and provides a versatile analytical framework for CRISPR-powered electrochemical nucleic acid detection.

Indexed as

Acinetobacter baumanniiBiosensing TechniquesElectrochemical TechniquesNucleic Acid Amplification TechniquesRecombinasesStaphylococcus aureusDNA, BacterialDNA, CircularHumansLimit of DetectionPhosphorylationDNA, BacterialDNA, CircularRecombinasesCRISPR/Cas12aElectrochemical biosensorPhosphorylation-asymmetric RPARolling circle amplificationTarget-directed circularization

Identifiers

PMID42814255

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.