Evidence map›Paper›PMID 42811315›Full record

ArticleCancer cell international2026

Cold atmospheric plasma treated medium (PTM) induce antitumor effects in human ovarian cancer cells.

Franziska Keßler, Franziska Oelkrug, Ernst Oberlechner, Jürgen Andress, Sara Y Brucker, André Koch, Daniel A Carvajal Berrio, Katja Schenke-Layland, Martin Weiss

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Article in Cancer cell international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Franziska Keßler *Department of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany.
Franziska Oelkrug *Department of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany.
Ernst OberlechnerDepartment of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany.
Jürgen AndressDepartment of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany.
Sara Y BruckerDepartment of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany.
André KochDepartment of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany.
Daniel A Carvajal BerrioInstitute of Biomedical Engineering, Eberhard Karls University, 72076, Tübingen, Germany.
Katja Schenke-LaylandNMI Natural and Medical Sciences Institute, 72770, Reutlingen, Germany.
Martin WeissDepartment of Women's Health, Eberhard Karls University, 72076, Tübingen, Germany. martin.weiss@med.uni-tuebingen.de.

Funding

Deutsche Forschungsgemeinschaft GRK 2543/1Faculty of Medicine of the Eberhard Karls University Tübingen 243210 and 41700German Research Foundation GRK 2543/1German Research Foundation INST 2388/341 and INST 2388/641Martin Weiss, Faculty of Medicine of the Eberhard Karls University Tübingen IZKF 2018106
6 · The paper itself

Abstract

Plasma treated solutions (PTS) have emerged as a promising anticancer strategy due to their ability to generate reactive oxygen and nitrogen species (RONS) that induce oxidative stress and disrupt multiple cellular pathways. Despite increasing interest in plasma-based cancer therapies, the mechanisms underlying their effects in ovarian cancer remain incompletely understood. This study aimed to investigate the biological effects of plasma treated medium (PTM) in ovarian cancer models, focusing on cell viability, caspase 3/7 activity, DNA damage, cell cycle progression, and mitochondrial metabolism.

methodsThree established ovarian cancer cell lines were treated with PTM to evaluate dose-dependent effects on cell viability using a resazurin assay. Apoptotic signaling was assessed by caspase 3/7 activity assays. DNA damage was analyzed by immunofluorescence staining of γH2AX, while cell cycle alterations were evaluated using propidium iodide staining and flow cytometry. Mitochondrial metabolism was investigated using fluorescence lifetime imaging microscopy together with NADH and FAD autofluorescence imaging. In addition, patient-derived high-grade serous ovarian cancer cells isolated from ascites were analyzed as independent biological replicates to assess biological relevance.

resultsPTM induced dose-dependent inhibition of cell viability with IC

conclusionsPTM exerts multifactorial anticancer effects in ovarian cancer models by inducing DNA damage, caspase 3/7 activity, cell cycle alterations, and mitochondrial dysfunction. These findings provide further insight into the biological effects of plasma-based cancer therapies and support the potential of plasma treated solutions as a novel therapeutic strategy for ovarian cancer.

Indexed as

Anticancer therapyAscitesDNA damageLow thermal argon plasma devitalizationMitochondrial dysfunctionNoninvasive physical plasmaOvarian cancerPlasma treated medium

Identifiers

PMID42811315
PMCPMC13625382

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.