ArticleEmerging infectious diseases2026
Detection of Influenza D Virus using Reverse Transcription Loop-Mediated Isothermal Amplification.
Article in Emerging infectious diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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11 authors.
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Abstract
The Orthomyxoviridae family includes influenza D virus (IDV), an emerging pathogen primarily affecting cattle and swine; there is evidence of cross-species transmission and potential zoonotic risk. Although active human infections have yet to be confirmed, high seroprevalence in cattle-exposed populations highlights the need for continued surveillance. We developed and validated a rapid, field-deployable reverse transcription loop-mediated isothermal amplification assay for IDV detection; specificity was 99.2% and sensitivity ranged from 95.6% (cycle quantification <30) to 81.8% (cycle quantification <40). This method offers a cost-effective, accessible alternative to quantitative reverse transcription PCR, enabling improved monitoring of IDV and reinforcing preparedness for emerging influenza threats.
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