Evidence map›Paper›PMID 42811066›Full record

ArticleCommunications biology2026

Cryo-EM structures of CENP-A nucleosomes isolated from human cells.

Eisho Tanida, Suguru Hatazawa, Takumi Oishi, Yuki Kobayashi, Mitsuo Ogasawara, Marta A Jaskiewicz, Akihito Harada, Takeru Fujii, Yasuyuki Ohkawa, Yoshimasa Takizawa and 1 more

Abstract read
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In one paragraph

Article in Communications biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Eisho TanidaLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Suguru HatazawaLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.ORCID 0000-0002-5678-4049
Takumi OishiLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Yuki KobayashiLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Mitsuo OgasawaraLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.
Marta A JaskiewiczLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.ORCID 0009-0000-6260-9952
Akihito HaradaDepartment of Multi-Omics, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.ORCID 0009-0003-1420-4549
Takeru FujiiDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.ORCID 0009-0004-5606-721X
Yasuyuki OhkawaDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.ORCID 0000-0001-6440-9954
Yoshimasa TakizawaLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan.ORCID 0000-0002-6265-4830
Hitoshi KurumizakaLaboratory of Chromatin Structure and Function, Institute for Quantitative Biosciences, The University of Tokyo, Tokyo, Japan. kurumizaka@iqb.u-tokyo.ac.jp.ORCID 0000-0001-7412-3722

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Centromeres ensure accurate chromosome segregation, and their identity is specified epigenetically by the histone H3 variant CENP-A. Although the structures of reconstituted CENP-A nucleosomes have provided important insights, how this epigenetic mark is structurally organized within native chromatin remains incompletely understood. Here, we combine cryogenic electron microscopy with chromatin immunoprecipitation (ChIP-CryoEM) to determine the structures of human CENP-A nucleosomes isolated directly from cells without crosslinking or stabilizing agents. We resolved two distinct cellular CENP-A nucleosome structures: a homotypic CENP-A nucleosome and a heterotypic CENP-A/H3 nucleosome, which have been proposed to represent centromeric and ectopic nucleosomes, respectively. Both cellular CENP-A nucleosomes assemble as histone octamers and wrap DNA in a left-handed superhelix, supporting an octameric model proposed for native CENP-A chromatin. In addition, the hallmark CENP-A-specific structural features previously characterized in vitro, including the shortened CENP-A N-terminal α-helix and the distinctive RG loop, are preserved in cellular CENP-A nucleosomes. These findings define the structural organization of CENP-A nucleosomes in cellular chromatin.

Indexed as

Centromere Protein ACryoelectron MicroscopyNucleosomesCentromereChromatin ImmunoprecipitationHistonesHumansCENPA protein, humanCentromere Protein AHistonesNucleosomes

Identifiers

PMID42811066
PMCPMC13623831

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.