ArticleBiotechnology letters2026
Transcriptome sequencing of Bacillus sp. HMM and effect of GH gene overexpression on cellulase activities.
Lanqian Su Shisong Xiyao Jianmei Qing Huili HuangLiangYuWangOuWangHuang, Chuzun Liao, Shiru Huang, Huiqing Zhang, Changhua Shang
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In one paragraphArticle in Biotechnology letters, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Lanqian Su Shisong Xiyao Jianmei Qing Huili HuangLiangYuWangOuWangHuang *Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, 541006, China.
Chuzun LiaoKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, 541006, China.
Shiru HuangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, 541006, China.
Huiqing ZhangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, 541006, China.
Changhua ShangKey Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin (Guangxi Normal University) & University Engineering Research Center of Bioinformation and Genetic Improvement of Specialty Crops, Guangxi, Guilin, 541006, China. shangchanghua@mailbox.gxnu.edu.cn.ORCID https://orcid.org/0000-0003-1447-6117 Funding
Guangdong Provincial Key Laboratory of Renewable Energy Foundation E539kf1301Innovation Project of Guangxi Graduate Education XYCS2025111
6 · The paper itselfAbstract
Bacillus sp. HMM can efficiently yield cellulase. However, transcriptome data and sequences of important genes such as cellulase gene are indistinct now, which restricts genetic reconstruction of strain HMM. In current paper, GO, COG and KEGG classification of unigenes from transcriptome sequencing of strain HMM was analyzed in detail, and cellulase gene GH (TR1834) encoding glycosylhydrolase was selected. Moreover, transgenic Bacillus sp. HMM overexpressing GH (GHT) with recombinant plasmid PHY-300PLK-GH was constructed. Avicel activity, CMC-Na activity and filter paper activity of GH transgenic HMM (GHT) were 1.4-, 1.2- and 1.08-fold those of control Bacillus sp. HMM with empty plasmid PHY-300PLK. This study enhanced application value of Bacillus for hydrolysis of cellulose.
Indexed as
BacillusBacterial ProteinsCellulaseTranscriptomeCelluloseGene Expression ProfilingBacterial ProteinsCellulaseCelluloseBacillus sp. HMMEnzymatic activityGH geneTranscriptome
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