ArticleOral health & preventive dentistry2026
Casearia sylvestris Derivatives Inhibit Inflammation and Oxidative Activity and Promote Healing in Human Gingival Fibroblasts - An In Vitro Study.
Article in Oral health & preventive dentistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeNew promising adjuvant approaches for managing periodontal inflammation are emerging. Casearia sylvestris, a medicinal plant rich in diterpenes, has demonstrated anti-inflammatory, antioxidant, and reparative properties. The present study aimed to investigate the anti-inflammatory, antiproteolytic, and pro-healing effects of C. sylvestris leaf derivatives: ethanolic extract (extract), diterpene-enriched fraction (F2), and isolated casearin J (Cas J) on human gingival fibroblasts (HGF). METHODS AND MATERIALS: HGF were exposed to Escherichia coli lipopolysaccharide (LPS) and treated with C. sylvestris derivatives. Transcription and protein levels of TNF-α, IL-8, MMP-1, and iNOS were assessed by real-time polymerase chain reaction (PCR) and ELISA, respectively. Nitric oxide (NO) levels were measured using the Griess reaction. NF-κB p65 nuclear translocation and ERK1 phosphorylation were analysed by immunofluorescence and immunoassay, respectively. Cell viability and scratch wound-healing assays were also performed.
resultsE. coli LPS triggered a significant pro-inflammatory and proteolytic cellular response, characterised by increased TNF-α, IL-8, MMP-1, iNOS, and NO levels. Treatment with C. sylvestris derivatives significantly reduced the levels of these mediators, while also inhibiting NF-κB p65 translocation and ERK1 phosphorylation. All compounds were biocompatible and promoted cell migration and wound closure in vitro.
conclusionThese findings suggest that C. sylvestris leaf derivatives exert anti-inflammatory, antiproteolytic, and pro-healing effects, thus highlighting their potential as adjunctive phytotherapeutic agents for periodontal disease management.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.