Fabrication of thermoplastic microfluidic probes for nanospray desorption electrospray ionization mass spectrometry.

Renkai Dong, Anthony H Choi, Xiangtang Li, Xindi Tang, Li-Xue Jiang, Julia Laskin

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Article in International journal of mass spectrometry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

6 authors.

Renkai DongJames Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.

Anthony H ChoiJames Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.

Xiangtang LiJames Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.

Xindi TangJames Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.

Li-Xue JiangJames Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.

Julia LaskinJames Tarpo Jr. and Margaret Tarpo Department of Chemistry, Purdue University, West Lafayette, IN, 47907, United States.ORCID 0000-0002-4533-9644

Funding

Correlative and multimodal molecular imaging of biological tissues with high sensitivity and cellular spatial resolutionR01GM157351 · NIGMS · PURDUE UNIVERSITY · PI Julia Laskin · 2026 to 2026

$304k

NIGMS NIH HHS R01 GM157351

6 · The paper itself

Abstract

Nanospray desorption electrospray ionization mass spectrometry (nano-DESI MS) uses specially designed probes to achieve a stable liquid bridge on the sample surface, ensuring consistent analyte extraction. Here, we present a mold-based fabrication protocol for cyclic olefin copolymer (COC) microfluidic probes (MFPs). We describe mold design, probe fabrication, and probe setup for nano-DESI MS. Four mold fabrication methods, including computer numerical control (CNC) machining, metal 3D printing, photolithography, and selective laser-assisted etching (SLE), were evaluated, with SLE providing the highest structural precision and enabling reproducible channel fabrication. Performance evaluation using lysophosphatidylethanolamine (LPE) 17:1 standard demonstrated stable liquid bridge formation, clear spatial separation, reproducible signal response, and low background interference. This mold-based fabrication strategy produces reproducible, low-cost probes and offers an accessible approach for nano-DESI MS.

Indexed as

Ambient ionizationCyclic olefin copolymer (COC)Liquid bridgeMass spectrometry imagingMicrofluidic probeMold imprintingNano-DESIThermal bonding

Identifiers

PMID42808028

DOI10.1016/j.ijms.2026.117672

PMCPMC13618711

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.