Evidence map›Paper›PMID 42807853›Full record

ArticleFrontiers in cellular and infection microbiology2026

Loss of IL10RA rewires epithelial immune responses to intracellular

Umesh K Shandilya, Yan Liang, Heba Atalla, Bonnie Mallard, Niel A Karrow

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Umesh K ShandilyaDepartment of Animal Biosciences, University of Guelph, Guelph, ON, Canada.
Yan LiangCollege of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Heba AtallaDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Bonnie MallardDepartment of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, ON, Canada.
Niel A KarrowDepartment of Animal Biosciences, University of Guelph, Guelph, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Methods: CRISPR/Cas9-generated IL10RA knockout (KO) MAC-T bovine mammary epithelial cells (MECs), alongside wild-type (WT) controls, were infected with either Results: WT cells mounted markedly divergent responses to PS (8 DEGs) versus SCV (461 DEGs) infection, with no overlap between the two gene sets, indicating strain-specific engagement of distinct molecular programmes. SCV infection of WT cells up-regulated lipid and sterol biosynthetic pathways while suppressing epithelial barrier integrity genes ( Discussion: These findings establish IL10RA as a broad transcriptional homeostasis regulator in bovine MECs during staphylococcal infection, with disproportionate effects on inflammatory and barrier-related gene programmes under SCV challenge. The identified candidate genes warrant validation in primary bovine mammary cells, milk somatic cells, or clinical mastitis samples to confirm their relevance to chronic and recurrent disease pathogenesis.

Indexed as

Epithelial CellsInterleukin-10 Receptor alpha SubunitMammary Glands, AnimalStaphylococcal InfectionsStaphylococcus aureusAnimalsCattleCRISPR-Cas SystemsFemaleGene Expression ProfilingGene Knockout TechniquesMastitis, BovineInterleukin-10 Receptor alpha Subunitbovine mastitisCRISPR/Cas9IL10RAMAC-T cellsRNA-Seqsmall colony variant (SCV)Staphylococcus aureus

Identifiers

PMID42807853
PMCPMC13617740

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.