Evidence map›Paper›PMID 42807609›Full record

ArticleJournal of biomedical optics2026

Label-free two-photon fluorescence lifetime imaging of FADH

Shiqi Wang, Yixuan Yang, Chaodong Yin, Jiaqing Guo, Yan Xu, Binglin Shen, Shijun Huang, Li Wang, Jie Chen, Shuai Ye and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Shiqi WangShenzhen University, College of Physics and Optoelectronic Engineering, State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen, China.
Yixuan YangShenzhen University, College of Physics and Optoelectronic Engineering, State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen, China.
Chaodong YinBeijing XingYun Digital Technology Co., Ltd., Beijing, China.
Jiaqing GuoShenzhen University, College of Physics and Optoelectronic Engineering, State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen, China.
Yan XuBeijing XingYun Digital Technology Co., Ltd., Beijing, China.
Binglin ShenShenzhen University, College of Physics and Optoelectronic Engineering, State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen, China.
Shijun HuangBeijing XingYun Digital Technology Co., Ltd., Beijing, China.
Li WangShenzhen University, School of Artificial Intelligence, National Engineering Laboratory for Big Data System Computing Technology, Shenzhen, China.
Jie ChenShenzhen University, School of Artificial Intelligence, National Engineering Laboratory for Big Data System Computing Technology, Shenzhen, China.
Shuai YeShenzhen University, College of Physics and Optoelectronic Engineering, State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen, China.
Zhang HongEdan Instruments, Inc., Shenzhen, China.
Jianqiang LiShenzhen University, School of Artificial Intelligence, National Engineering Laboratory for Big Data System Computing Technology, Shenzhen, China.
Liwei LiuShenzhen University, College of Physics and Optoelectronic Engineering, State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen, China.ORCID https://orcid.org/0000-0002-4593-665X
Lingchao ChenHuashan Hospital Department of Neurosurgery, Fudan University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Significance: Glioma diagnosis currently relies on histopathology, which provides essential morphological information but fails to capture the dynamic metabolic heterogeneity underlying tumor progression. Aim: This study seeks to establish a label-free fluorescence lifetime imaging strategy to characterize the metabolic microenvironment of gliomas, with the goal of achieving accurate tumor grading and real-time malignancy assessment. Approach: We developed a phasor plot-based two-photon fluorescence lifetime imaging microscopy (TP-FLIM) system. This imaging approach was utilized to evaluate tumor tissues by mapping microenvironmental phenotypes across four distinct glioma subtypes. In addition, we performed longitudinal Results: This method successfully identified distinct tissue regions defined as reduced flavin adenine dinucleotide (FADH Conclusions: These findings establish FADH

Indexed as

Brain NeoplasmsFlavin-Adenine DinucleotideGliomaMicroscopy, Fluorescence, MultiphotonAnimalsCell Line, TumorHumansMiceNeoplasm GradingTumor MicroenvironmentFlavin-Adenine Dinucleotidefluorescence lifetime microscopy imaginggliomaoxidation-reduction reactionspathological diagnosis

Identifiers

PMID42807609
PMCPMC13617214

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.