ArticleIrish veterinary journal2026
Rapid on-site detection of Pasteurella multocida in cattle using an LFS-based RPA-CRISPR/Cas12a assay.
Article in Irish veterinary journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundPasteurella multocida is an important bacterial pathogen associated with bovine respiratory disease (BRD), causing substantial economic losses in cattle production worldwide. Rapid field detection of P. multocida remains challenging because conventional diagnostic methods are often time-consuming and require specialized laboratory equipment. This study aimed to establish a rapid visual assay for P. multocida to support field diagnosis and disease management in cattle.
methodsA recombinase polymerase amplification (RPA)-CRISPR/Cas12a assay targeting the species-specific kmt1 gene of P. multocida was developed and combined with lateral flow strips (LFS) for instrument-free visual detection. The assay performance was comprehensively evaluated based on sensitivity, specificity, repeatability, and validation using clinical samples.
resultsUnder optimized conditions, the RPA amplification was completed within 15 min at 37 °C, and the CRISPR/Cas12a reaction generated clear visual signals on the LFS. The assay achieved a detection limit of 10
conclusionsThe established LFS-based RPA-CRISPR/Cas12a assay enables rapid, sensitive, and instrument-independent detection of P. multocida. This method provides a practical point-of-care testing (POCT) tool for rapid on-site screening of P. multocida in resource-limited veterinary settings.
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