Evidence map›Paper›PMID 42806333›Full record

ArticleRespiratory research2026

The Tie-2 agonist PMC-403 inhibits endothelial damage induced by e-cigarette vapor or LPS.

Thorben Pape, Oleg Pak, Theresa Graalmann, Christine Ehlers, Janik Deutscher, David Spiegelberg, Paul Horn, Klaus Stahl, Marius M Hoeper, Sascha David and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Respiratory research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Thorben Pape *Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Carl-Neuberg-Str.1, Hannover, 30625, Germany. pape.thorben@mh-hannover.de.
Oleg Pak *Excellence Cluster Cardio-Pulmonary Institute, University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Institute for Lung Health (ILH), Justus Liebig University Giessen, Giessen, Germany.
Theresa GraalmannBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany.
Christine EhlersBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany.
Janik DeutscherExcellence Cluster Cardio-Pulmonary Institute, University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Institute for Lung Health (ILH), Justus Liebig University Giessen, Giessen, Germany.
David SpiegelbergExcellence Cluster Cardio-Pulmonary Institute, University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Institute for Lung Health (ILH), Justus Liebig University Giessen, Giessen, Germany.
Paul HornDepartment of Hepatology and Gastroenterology, Campus Virchow-Klinikum (CVK) and Campus Charité Mitte (CCM), Charité - Universitätsmedizin Berlin, Berlin, Germany.
Klaus StahlDepartment of Gastroenterology, Hepatology, Infectious Diseases and Endocrinology, Hannover Medical School, Hannover, Germany.
Marius M HoeperDepartment of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Carl-Neuberg-Str.1, Hannover, 30625, Germany.
Sascha DavidInstitute of Intensive Care Medicine, University Hospital Zürich and University of Zurich, Zurich, Switzerland.
Natascha Sommer *Excellence Cluster Cardio-Pulmonary Institute, University of Giessen and Marburg Lung Center (UGMLC), German Center for Lung Research (DZL), Institute for Lung Health (ILH), Justus Liebig University Giessen, Giessen, Germany.
Benjamin Seeliger *Department of Respiratory Medicine and Infectious Diseases, Hannover Medical School, Carl-Neuberg-Str.1, Hannover, 30625, Germany. seeliger.benjamin@mh-hannover.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe use of e-cigarettes is currently increasing despite potential harmful effects of e-cigarettes. Acute e-cigarette exposure may cause endothelial damage thereby contributing to acute lung injury or vascular dysfunction after chronic use. These effects may be enhanced by concomitant inflammatory stimuli. We investigated the acute effect of e-cigarette vapor (ECV) with or without concomitant lipopolysaccharide (LPS) stimulation on endothelial cell function in vitro and on vascular permeability in isolated lungs. Further potentially protective effects of the Tie2 agonist PMC-403 were studied.

methodsHuman umbilical cord endothelial cells (HUVECs) were cultured with different types of ECV extracts (ECVE) and/or LPS, and endothelial activation was determined in vitro by measuring secreted protein and mRNA levels of angiopoietin-2 (Angpt-2) and soluble Tie2 (sTie2). Barrier dysfunction was analyzed by continuous transendothelial electrical resistance (TER) measurements using electric cell-substrate impendence sensing (ECIS). An ex vivo isolated mouse lung model was used to analyze endothelial permeability and edema formation upon intratracheal ECV exposure. The Tie2 agonist PMC-403 was applied in vitro and ex vivo and its effect on endothelial permeability was determined.

resultsAngpt-2 secretion and transcription were significantly increased in HUVECs after 24h stimulation with nicotine-containing ECVE (ECVE

conclusionsECV

Indexed as

E-Cigarette VaporElectronic Nicotine Delivery SystemsEndothelium, VascularHuman Umbilical Vein Endothelial CellsLipopolysaccharidesPyridinesReceptor, TIE-2Angiopoietin-2AnimalsCapillary PermeabilityCells, CulturedHumansMaleMiceMice, Inbred C57BLAngiopoietin-2E-Cigarette VaporLipopolysaccharidesPyridinesReceptor, TIE-2TEK protein, humanARDSCapillary leakageECVECVESystemic inflammation

Identifiers

PMID42806333
PMCPMC13621675

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.