Evidence map›Paper›PMID 42806153›Full record

ArticleAnalytical and bioanalytical chemistry2026

CHA-mediated split G-quadruplex assembly: a ratiometric AIE strategy for label-free detection of APE1.

Chan Yang, Zihan Yan, Yanru Qin, Ying Wei, Ziyi He, Liuting Mo, Weiying Lin

Abstract read
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In one paragraph

Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chan YangInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China. yangchan361@163.com.
Zihan YanInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China.
Yanru QinInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China.
Ying WeiInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China.
Ziyi HeInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China.
Liuting MoInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China.
Weiying LinInstitute of Optical Materials and Chemical Biology, Guangxi Key Laboratory of Electrochemical Energy Materials, College of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi, 530004, P. R. China. weiyinglin2013@163.com.

Funding

National Natural Science Foundation of China 22204024National Natural Science Foundation of China 22277014National Natural Science Foundation of China 22404030National Natural Science Foundation of China 22577016Natural Science Foundation of Guangxi Zhuang Autonomous Region 2021GXNSFDA075003Natural Science Foundation of Guangxi Zhuang Autonomous Region 2022GXNSFBA035482Natural Science Foundation of Guangxi Zhuang Autonomous Region 2026GXNSFAA00640463Natural Science Foundation of Guangxi Zhuang Autonomous Region AD21220061Natural Science Foundation of Guangxi Zhuang Autonomous Region AD23026156the startup fund of Guangxi University A3040051003the startup fund of Guangxi University A3040051016
6 · The paper itself

Abstract

Apurinic/apyrimidinic endonuclease 1 (APE1), a critical enzyme in the base excision repair pathway, is a potential biomarker for numerous cancer types. Traditional APE1 activity detection methods are hindered by complex protocols, expensive labeling requirements, and vulnerability to environmental factors. Herein, we develop a label-free ratiometric fluorescent biosensor (TCHG) that integrates APE1-mediated catalytic hairpin assembly (CHA) amplification with split G-quadruplex/malachite green (MG)-mediated aggregation-induced emission (AIE) signaling for sensitive activity analysis of APE1. Our system operates through APE1's specific cleavage of apurinic/apyrimidinic (AP) site-containing double-stranded substrates, which releases trigger strands that initiate a CHA cascade. This cascade drives the assembly of split G-quadruplex structures. Upon reconstruction, the complete G-quadruplex binds MG, generating amplified near-infrared fluorescence with AIE characteristics. Simultaneously, Hoechst 33258 (HOE) embedded within double-stranded DNA provides a stable reference signal, enabling self-calibrated ratiometric output that effectively minimizes signal fluctuations from instrumental and environmental variations. The TCHG biosensor demonstrates high selectivity for APE1 with a detection limit of 2.4 × 10

Indexed as

Aggregation-induced emissionCatalytic hairpin assemblyLabel-freeRatiometric fluorescenceSplit G-quadruplex

Identifiers

PMID42806153

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.