Profiling extracellular vesicle glycoRNAs defines their function in vesicular communication.

Ximing Chen, Chaojie Hao, Xiaodie Chen, Ting Chen, Weirong Xiao, Jiaheng Zhu, Jialu Zhang, Yuqian Zhang, Mingying Chen, Hongyu Lin and 1 more

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Article in Nature structural & molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Ximing Chen *Fujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Chaojie Hao *Fujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Xiaodie Chen *Fujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Ting Chen *Fujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Weirong Xiao *Fujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Jiaheng ZhuInstitute of Electromagnetics and Acoustics and Key Laboratory of Electromagnetic Wave Science and Detection Technology, Xiamen University, Xiamen, China.

Jialu ZhangFujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Yuqian ZhangFujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Mingying ChenFujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Hongyu LinFujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China.

Yanling SongFujian Key Laboratory of Chemical Biology (Xiamen University), The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, China. ylsong@xmu.edu.cn.ORCID http://orcid.org/0000-0002-6793-6685

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Glycosylated RNAs (glycoRNAs) reside on the cell surface with their functions largely unexplored. Here, we show that extracellular vesicles (EVs) carry glycoRNAs with distinct sequence diversity, vesicle type specificity and transfer dynamics. By metabolically tagging glycoRNAs with bioorthogonal handles, we selectively enrich and sequence EV-associated glycoRNAs, identifying specific glycoRNA species and expanding the EV glycoRNA landscape. We further develop a proximity-based imaging strategy to track glycoRNAs, revealing that glycoRNA repertoires in large EVs vary with cell type and passage, whereas small EVs display distinct profiles indicative of vesicle-specific sorting. Functionally, EVs deliver glycoRNAs into recipient cells and glycoRNAs are subsequently detected in recipient cell-derived EVs, reflecting contributions from newly synthesized glycoRNAs and others that may be associated with donor EVs. Notably, hybridization-based glycoRNA blockade can heavily reduce EV uptake, highlighting glycoRNAs as potentially actionable targets for modulating EV-cell communication. These findings expand the glycoRNA landscape and suggest EV glycoRNAs are biomarkers of cell state and regulators of vesicle tropism.

Identifiers

PMID42806047

DOI10.1038/s41586-018-0392-8

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.