Evidence map›Paper›PMID 42804096›Full record

ArticleBulletin of experimental biology and medicine2026

Generation of Human Cells with Monoallelic Expression of HLA‑A in HLA Class I Knockout Cells for Evaluation of Antigen Presentation.

U Boyarskikh, A Serych, E Khrapov, D Shamovskaya, M Filipenko

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Article in Bulletin of experimental biology and medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

U BoyarskikhInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia. boyarskih.u@gmail.com.
A SerychInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
E KhrapovInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
D ShamovskayaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
M FilipenkoInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cells expressing monoallelic HLA class I molecules represent a convenient in vitro model for studying the immunogenicity of tumor protein neoepitopes. Such models can be generated using endogenous HLA class I-deficient cells. Here, we describe an effective method for eliminating HLA class I expression in HEK293T cells via simultaneous CRISPR/Cas9 editing of the HLA‑A, HLA‑B, and HLA‑C genes using a single guide RNA. An important advantage of this method is the possibility to maintain the unedited HLA‑E gene. The antigen-presenting function of the resulting cells was successfully restored by transduction of a single HLA class I allele. Cells expressing monoallelic HLA‑A, after processing SARS-CoV‑2 epitopes, induced the allele-specific immune response in leukocytes isolated from patients who had recovered from COVID-19. Thus, the obtained HLA class I-knockout cells can be used to evaluate allele-specific neoantigen presentation.

Indexed as

antigen presentationCRISPR/Cas9HLA class I

Identifiers

PMID42804096

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.