Evidence map›Paper›PMID 42801749›Full record

ReviewChembiochem : a European journal of chemical biology2026

Nanorulers for Super-Resolution Microscopy.

Gerti Beliu, Alan M Szalai, Guillermo Acuna, Alexey Chizhik, José Ignacio Gallea, Jörg Enderlein

Abstract readReview
In one paragraph

Review in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Gerti BeliuFaculty of Chemistry and Pharmacy-Bioimaging, Regensburg Ultrafast Nanoscopy (RUN), University of Regensburg, Regensburg, Germany.
Alan M SzalaiDépartement de Physique - Photonic Nanosystems, Université de Fribourg - Faculté des Sciences et de Médecine, Fribourg, Switzerland.
Guillermo AcunaDépartement de Physique - Photonic Nanosystems, Université de Fribourg - Faculté des Sciences et de Médecine, Fribourg, Switzerland.ORCID https://orcid.org/0000-0001-8066-2677
Alexey ChizhikThird Institute of Physics (Biophysics), Georg August University, Göttingen, Germany.
José Ignacio GalleaThird Institute of Physics (Biophysics), Georg August University, Göttingen, Germany.ORCID https://orcid.org/0000-0002-9762-1203
Jörg EnderleinThird Institute of Physics (Biophysics), Georg August University, Göttingen, Germany.ORCID https://orcid.org/0000-0001-5091-7157

Funding

Deutsche Forschungsgemeinschaft EXC 2067/1-390729940European Research Council 884488
6 · The paper itself

Abstract

Super-resolution fluorescence microscopy has advanced into a regime where molecular-scale organization can be resolved with nanometer precision. In this context, the accurate interpretation of imaging data increasingly depends on well-defined calibration standards. Nanorulers have emerged as essential tools to validate resolution, quantify labeling performance, and benchmark analytical methods. Current approaches include DNA-based, protein-based, and virus-based nanorulers, each offering distinct advantages in terms of programmability, structural definition, and biological relevance. This minireview provides an overview of these complementary strategies, with a particular focus on recent developments in protein-based nanorulers enabled by advances in protein engineering and site-specific labeling. Together, these approaches contribute to establishing reliable calibration frameworks for quantitative molecular bioimaging.

Indexed as

DNAProteinsFluorescent DyesHumansMicroscopy, FluorescenceProtein EngineeringSingle Molecule ImagingDNAFluorescent DyesProteinsnanorulerssingle‐molecule localization microscopysuper‐resolution microscopy

Identifiers

PMID42801749
PMCPMC13616436

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.