ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Thermal Shifts Dominate Single-Gene Responses to Transcription-Targeting Antibiotics.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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6 authors.
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Abstract
Combined exposure to thermal and antibiotic stresses is ubiquitous. How cells adapt to these concurrent cues at the single-gene level remains unresolved. Here, we show that in Escherichia coli, both cold and heat shock responses dominate antibiotic stress responses across multiple levels of physiology. Using RNA-seq, we demonstrate that combined exposure produces transcriptomes that are largely indistinguishable from those induced by thermal shifts alone. Flow-cytometry measurements across intermediate temperatures and increasing ofloxacin concentrations further show that temperature-like responses are more prevalent outside the 30°C-39°C range. This dominance arises from temperature-driven changes in ATP levels and nucleoid organization, which in turn modulate DNA gyrase and RNA polymerase engagement with DNA. Combined exposure also induces SoxS- and MarA-mediated efflux systems more strongly than individual stresses. Machine learning analysis identifies CRP and σ
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