Evidence map›Paper›PMID 42801547›Full record

ArticlePathology international2026

Digital PCR for Detecting Loss of BAP1 and MTAP in Pleural Fluids of Mesothelioma Patients: Perspectives in the Diagnosis.

Giulia Rosini, Greta Alì, Rossella Bruno, Monica Cipollini, Rudy Foddis, Alessandra Celi, Roberto Silvestri, Filomena Rea, Vittorio Aprile, Marco Lucchi and 2 more

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In one paragraph

Article in Pathology international, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Giulia RosiniGenetics-Department of Biology, University of Pisa, Pisa, Italy.ORCID https://orcid.org/0000-0001-8863-4670
Greta AlìUnit of Pathological Anatomy, University of Pisa, Pisa, Italy.ORCID https://orcid.org/0000-0001-9258-3466
Rossella BrunoUnit of Pathological Anatomy, University of Pisa, Pisa, Italy.
Monica CipolliniGenetics-Department of Biology, University of Pisa, Pisa, Italy.ORCID https://orcid.org/0009-0008-4980-0566
Rudy FoddisDepartment of Translational Research and of New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.ORCID https://orcid.org/0000-0003-1330-3863
Alessandra CeliUnit of Pathological Anatomy, University of Pisa, Pisa, Italy.
Roberto SilvestriGenetics-Department of Biology, University of Pisa, Pisa, Italy.ORCID https://orcid.org/0000-0002-5588-5235
Filomena ReaGenetics-Department of Biology, University of Pisa, Pisa, Italy.
Vittorio AprileCardiac, Thoracic, and Vascular Department, Division of Thoracic Surgery, University Hospital of Pisa, Pisa, Italy.ORCID https://orcid.org/0000-0001-8538-1719
Marco LucchiCardiac, Thoracic, and Vascular Department, Division of Thoracic Surgery, University Hospital of Pisa, Pisa, Italy.
Federica GemignaniGenetics-Department of Biology, University of Pisa, Pisa, Italy.
Stefano LandiGenetics-Department of Biology, University of Pisa, Pisa, Italy.ORCID https://orcid.org/0000-0001-8364-6357

Funding

AIRC IG-25708European Union-NextGenerationEU grant through the Italian Ministry of University and Research under PNRR- National Center 3 - Spoke No. 2 -Development of Gene Therapy and RNA-Based Drugs I53C22000710007Italian Ministry of Universities and Research under the Department of Excellence 2023-2027 initiative
6 · The paper itself

Abstract

Pleural mesothelioma (PM) often presents loss of BAP1 and MTAP genes. Digital PCR (dPCR) can accurately measure gene copy numbers (CNs). We hypothesized that cell-free DNA (cfDNA) extracted from the supernatant of pleural fluids (SPFs) could allow an efficient detection of these genetic events. BAP1/MTAP CN was measured with dPCR in SPFs and in solid tumor biopsies (STBs) of 37 PM patients. Results were compared with immunohistochemistry (IHC) carried out in STBs. IHC alone detected BAP1/MTAP-losses in 32/37 (86.5%). However, the sensitivity improved to 34/37 (91.9%) when low CN of BAP1/MTAP was included. Among 20 patients presenting SPFs, dPCR alone detected a low CN in 18 samples (sensitivity = 90%). Finally, this two-gene signature was applied on SPFs in a prospective series of six consecutive patients presenting an undefined diagnosis (negative for PM) and two showed reduced CN. After 7 months of follow-up, these subjects were further re-evaluated for the persistence/worsening of the symptoms and were diagnosed for PM. We highlighted dPCR's potential for detecting subtle BAP1/MTAP losses in SPFs of PM patients. This appears as a complementary approach for a more accurate diagnosis in specific contexts, when high-risk patients receive negative results after IHC analysis.

Indexed as

MesotheliomaPleural NeoplasmsPolymerase Chain ReactionTumor Suppressor ProteinsUbiquitin ThiolesteraseAdultAgedBiomarkers, TumorFemaleGene DosageHumansMaleMesothelioma, MalignantMiddle AgedProspective StudiesPurine-Nucleoside Phosphorylase5'-methylthioadenosine phosphorylaseBAP1 protein, humanBiomarkers, TumorPurine-Nucleoside PhosphorylaseTumor Suppressor ProteinsUbiquitin ThiolesteraseBAP1digital PCRgene copy numberimmunohistochemistryliquid biopsyMTAPpleural fluidspleural mesothelioma

Identifiers

PMID42801547
PMCPMC13616427

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.