Evidence map›Paper›PMID 42801352›Full record

ArticleMolecular biology reports2026

Evaluation of 18 S rRNA as an endogenous control in OpenArray

Habib Francis, Andrzej S Januszewski, Abubakar Mangani, Fahmida K Ema, Anandwardhan A Hardikar, Mugdha V Joglekar, Michael L H Huang, David R Sullivan, Ronald C W Ma, Sanjeev Galande and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Habib FrancisSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia. Habib.Francis@sydney.edu.au.ORCID http://orcid.org/0009-0009-1106-4118
Andrzej S JanuszewskiSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.ORCID http://orcid.org/0000-0003-0995-4524
Abubakar ManganiSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.
Fahmida K EmaSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.
Anandwardhan A HardikarWestern Sydney University School of Medicine, Campbelltown, Australia.
Mugdha V JoglekarWestern Sydney University School of Medicine, Campbelltown, Australia.
Michael L H HuangSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.
David R SullivanRoyal Prince Alfred Hospital, Camperdown, Australia.ORCID http://orcid.org/0000-0003-3085-5627
Ronald C W MaDepartment of Medicine and Therapeutics and Li Ka Shing Institute of Health Sciences, Faculty of Medicine, Prince of Wales Hospital, The Chinese University of Hong Kong, Hong Kong, China.ORCID http://orcid.org/0000-0002-1227-803X
Sanjeev GalandeShiv Nadar Institution of Eminence, Tehsil Dadri, Gautam Buddha Nagar, Uttar Pradesh, India.
Val GebskiSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.
R John SimesSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.
Alicia J JenkinsSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.
Mark P MolloyBowel Cancer and Biomarker Research Laboratory, School of Medical Sciences, Kolling Institute, The University of Sydney, St Leonards, Australia.ORCID http://orcid.org/0000-0003-4679-5868
Anthony C KeechSydney Clinical Trials Centre, The University of Sydney, Sydney, Australia.

Funding

National Health and Medical Research Council of Australia 1147897
6 · The paper itself

Abstract

backgroundReliable qPCR analysis depends on stable endogenous controls, particularly in high-throughput pre-amplification workflows where technical bias may arise. The suitability of highly abundant 18 S rRNA as a reference gene in multiplex OpenArray

methodsPlasma RNA from the Fenofibrate Intervention and Event Lowering in Diabetes (FIELD) trial was analysed using TaqMan OpenArray

results18 S rRNA showed stable detection (median Ct 14.5, IQR 13.4-16.3) with low inter-run variability (CV 3.9%). Inclusion of 18 S rRNA during pre-amplification did not significantly alter Ct values of co-amplified targets (median differences 0.07-0.52 cycles; p > 0.05), with minimal overall bias (-0.18 Ct). Archived and newly manufactured primer pools showed a small but significant Ct shift, with lower values in the new pool (bias 0.53 Ct; p < 0.0001), although variability between paired measurements was wide.

conclusion18 S rRNA is a stable technical control in plasma OpenArray

Indexed as

Real-Time Polymerase Chain ReactionRNA, Ribosomal, 18SHumansReference StandardsReproducibility of ResultsRNAWorkflowRNARNA, Ribosomal, 18S18S rRNAEndogenous controlGene expressionOpenArrayPlasma RNAPre-amplificationqPCR

Identifiers

PMID42801352
PMCPMC13616856

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.