Evidence map›Paper›PMID 42801341›Full record

ArticlePlant cell reports2026

Comparative transcriptome profiling and functional analysis of GmRAMOSA1 as a key regulator of adventitious shoot regeneration in soybean.

Yuhan Liu, Xinxin Zhang, Hengke Jiang, Dewei Mu, Qingyu Qin, Weiting Wu, Yang Zhou, Yanpeng Zhang, Jun Gu, Hui Yang and 4 more

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Plant cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

14 authors.

Yuhan LiuCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Xinxin ZhangCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Hengke JiangCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Dewei MuCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Qingyu QinCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Weiting WuCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yang ZhouCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yanpeng ZhangCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jun GuCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Hui YangCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Jiaxian HeCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China.
Yi HuangOil Crops Research Institute of Chinese Academy of Agricultural Sciences, Wuhan, China.
Xin SunCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. sunxin529@163.com.
Junbo DuCollege of Agronomy, Sichuan Agricultural University, Chengdu, 611130, Sichuan, China. junbodu@sicau.edu.cn.

Funding

Sichuan Innovation Team Project of National Modern Agricultural Industry Technology System, China SCCXTD-2026-21Sichuan Science and Technology Program 2023YFN0083Sichuan Science and Technology Program 2024YFCY0010-01the National Natural Science Foundation of China 32171939the Open Project of Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, P. R. China KF2024006
6 · The paper itself

Abstract

key messageThis study identifies GmRAMOSA1 as a key regulator of adventitious shoot regeneration in soybean and uncovers its regulatory network via comparative transcriptome profiling, providing new insights into the molecular mechanism of soybean shoot organogenesis. Soybean genetic transformation is strongly constrained by genotype-dependent in vitro regeneration capacity, particularly in adventitious shoot formation. Here, we compared the highly regenerable cultivar Dongnong 50 (DN50) with the low regenerative capacity Sichuan landrace Gongqiudou 5 (GQD5) using a cotyledonary node-based adventitious shoot induction system. RNA sequencing (RNA-seq) was performed at multiple stages post-induction. Hormone-related pathways were significantly enriched, and genes involved in auxin and cytokinin signaling exhibited distinct temporal dynamics between DN50 and GQD5. Integration of co-expression network analysis and promoter motif enrichment prioritized GmRAMOSA1 (GmRA1) as a candidate regulator. Overexpression of GmRA1 in the GQD5 background significantly increased adventitious shoot regeneration and was associated with elevated expression of several meristem-identity and hormone-responsive genes. This study highlights a promising molecular target for enhancing soybean regeneration and transformation efficiency.

Indexed as

Glycine maxPlant ProteinsPlant ShootsRegenerationGene Expression ProfilingGene Expression Regulation, PlantIndoleacetic AcidsPlant Growth RegulatorsPlants, Genetically ModifiedTranscriptomeIndoleacetic AcidsPlant Growth RegulatorsPlant ProteinsAdventitious shoot regenerationComparative transcriptomeGenetic transformationGmRAMOSA1Soybean

Identifiers

PMID42801341

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