Evidence map›Paper›PMID 42799842›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2027

Cell Biology of Botrytis: Live-Cell Imaging Techniques and Protein Localization Protocols.

H M Suraj, André Fleissner, Jan A L van Kan

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

H M SurajLaboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands.ORCID http://orcid.org/0000-0002-5936-1420
André FleissnerInstitute for Genetics, Technische Universität Braunschweig, Braunschweig, Germany.
Jan A L van KanLaboratory of Phytopathology, Wageningen University & Research, Wageningen, The Netherlands. jan.vankan@wur.nl.ORCID http://orcid.org/0000-0003-3563-1550

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Botrytis cinerea is a widely studied model organism for plant-fungal interactions. This chapter provides simple and easy protocols for live-cell imaging techniques and subcellular protein localization studies in B. cinerea in vitro. Using a simple microscopy setup, we describe methods for imaging spore germlings and hyphae and for studying protein dynamics in response to the antifungal compound α-tomatine. The protocols highlight the application of fluorescence microscopy to visualize sterol distribution and protein localization patterns, enabling deeper insights into fungal cell biology.

Indexed as

BotrytisFungal ProteinsHyphaeMicroscopy, FluorescenceSpores, FungalFungal ProteinsBotrytis cinereaLive-cell imagingMicroscopyProtein localizationStaining

Identifiers

PMID42799842

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.