Evidence map›Paper›PMID 42797925›Full record

ArticleVeterinary sciences2026

Development and Application of a Triplex qPCR Assay for the Simultaneous Detection of African Swine Fever Virus, Porcine Circovirus Type 2, and Porcine Circovirus Type 3.

Shunying Huang, Xing Chang, Jin Cui, Chunju Liu, Yanli Zou, Weijie Ren, Xiuju Han, Shan Liu, Xiaojing Yu, Yunxia Wang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Veterinary sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Shunying HuangFaculty of Agriculture, Yanbian University, Yanji 133002, China.
Xing ChangChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Jin CuiChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Chunju LiuChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Yanli ZouChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Weijie RenChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Xiuju HanChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Shan LiuChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Xiaojing YuChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Yunxia WangChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Jinming LiChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Zhiliang WangChina Animal Health and Epidemiology Center, Qingdao 266032, China.
Jianchen SongFaculty of Agriculture, Yanbian University, Yanji 133002, China.

Funding

Qinghai Institute for Endemic Diease Prevention and Control 2026NK117
6 · The paper itself

Abstract

African swine fever virus (ASFV), porcine circovirus type 2 (PCV2), and porcine circovirus type 3 (PCV3) represent important viral agents that threaten pig health. To date, no triplex real-time quantitative PCR (qPCR) method has been described for the concurrent identification of all three viruses. In this study, a triplex qPCR system was constructed using the ASFV B646L gene, PCV2 ORF1 gene, and PCV3 ORF1 gene as amplification targets, enabling simultaneous detection of ASFV, PCV2, and PCV3. The standard curves generated for the three viral targets all yielded R

Indexed as

African swine fever virusmolecular detectionmultiplex real-time PCRporcine circovirus type 2porcine circovirus type 3

Identifiers

PMID42797925
PMCPMC13611774

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.