Bonding with a Benchtop UV Ozone Cleaner: A Practical Guide to Prepare a Microfluidic Chip.

This study describes a procedure for preparing microfluidic chips made from polydimethylsiloxane (PDMS) layers. It uses an Ossila UV ozone cleaner for surface activation instead of the more common oxygen plasma treatment. The process is limited to PDMS@PDMS bonding, and it can be used when a low-cost procedure for a small number of microfluidic chips is required or when an oxygen plasma etcher is unavailable. The challenges of this process arise from the slight hardening of the PDMS surface when it is activated for at least 70 min, which is necessary for reliable bonding. Activation of the surface is confirmed by contact angle measurements. In a static simulation and lab tests, it is demonstrated that damage resulting from this hardening in conjunction with careless handling of the microfluidic chip is mitigated by incorporating predetermined break structures and using tubes with an outer diameter that is smaller than the inlets. Additionally, pre-polymerized PDMS glue and PDMS seals are suggested to ensure that the tubes are properly sealed. A burst test in air was performed to demonstrate the stability of the bonding and gluing. IR and XPS measurements were performed to investigate surface and bulk changes due to the treatment. To demonstrate the concept, six microfluidic chips were prepared and tested, achieving flow rates of at least 170 µL/min at ±180 mbar. Bonding remains stable up to a pressure of approximately 428 ± 60 mbar.