Evidence map›Paper›PMID 42795456›Full record

ArticleMicroorganisms2026

Development and Validation of an Antigen-Capture ELISA for Quantitative Detection of Donkey-Origin Rotavirus: Application in Vaccine Manufacturing Process Monitoring.

Zenan Zhang, Xing Guo, Kui Guo, Wei Guo, Cheng Du, Yan Yang, Wenjing Dong, Xiaoyu Chu, Yuezhi Lin, Xiaojun Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microorganisms, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Zenan ZhangState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0002-6867-6598
Xing GuoState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0001-9496-2267
Kui GuoState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0001-6274-1857
Wei GuoState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0003-1507-737X
Cheng DuState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0002-1686-6189
Yan YangState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0002-3862-2003
Wenjing DongState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0009-0001-3974-0394
Xiaoyu ChuState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0001-6479-6659
Yuezhi LinState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0001-7281-0369
Xiaojun WangState Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150069, China.ORCID 0000-0003-4521-4099

Funding

Heilongjiang Provincial Natural Science Foundation of China LH2023C049Prevention and Control of Emerging and Major Infectious Diseases-National Science and Tech-nology Major Project 2025ZD01900100Xinjiang Talent Development Fund ZZYD2023010
6 · The paper itself

Abstract

Rotavirus A (RVA) causes severe diarrhea in donkey foals, but rapid quantitative antigen assays for vaccine development and manufacturing control are lacking. We developed a VP6-based antigen-capture ELISA (acELISA) for donkey-origin RVA using rabbit anti-RVA polyclonal IgG as the capture antibody and horseradish peroxidase-conjugated mouse anti-VP6 monoclonal antibody 3F6 as the detector antibody. Assay conditions were optimized, and performance was evaluated through standard-curve analysis, specificity and repeatability testing, Western blot (WB) comparison, median tissue culture infectious dose (TCID

Indexed as

acELISAdonkey rotavirustangential flow filtrationvaccine manufacturingVP6

Identifiers

PMID42795456
PMCPMC13609450

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.