Molecular Mechanisms of Programmed Cell Death During Somatic Embryo Detachment from Callus in Indirect Somatic Embryogenesis.

Haibing Huang, Zhongzheng Ma, Tiantian Sun, Fude Wang

Abstract readReview

In one paragraph

Review in International journal of molecular sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Haibing HuangHeilongjiang Institute of Wood Science, Harbin 150081, China.

Zhongzheng MaState Key Laboratory of Tree Genetics and Breeding, College of Forestry, Northeast Forestry University, Harbin 150040, China.

Tiantian SunState Key Laboratory of Tree Genetics and Breeding, College of Forestry, Northeast Forestry University, Harbin 150040, China.

Fude WangHeilongjiang Institute of Wood Science, Harbin 150081, China.ORCID 0009-0009-6909-4408

Funding

National Key Research and Development Plan of China 2022YFD2200303-3

6 · The paper itself

Abstract

Somatic embryogenesis in plants occurs via direct or indirect routes. In indirect somatic embryogenesis, embryos initiate from callus. The detachment of these embryos from callus is a critical step for plantlet recovery. Most reviews focus only on embryo induction. This review focuses specifically on the detachment stage. We examine how programmed cell death (PCD) drives this physical release. Cell death occurs in boundary callus cells and the suspensor. It does not destroy the developing embryo proper. PCD performs three main tasks. It dissolves middle lamella pectins. It removes the temporary suspensor. And it digests doomed cells to recycle nutrients for the embryo. Key proteases such as metacaspases execute this death program, with vacuolar processing enzymes representing candidate execution proteases. Epigenetic marks and transcription factors restrict cell death to the boundary. We also discuss practical ways to regulate PCD in tissue culture.

Indexed as

ApoptosisPlant Somatic Embryogenesis TechniquesSeedsPeptide HydrolasesTranscription FactorsPeptide HydrolasesTranscription Factorscell wall remodelingexecution proteasesin vitro regenerationLAFL transcription factorstissue abscissionvacuolar autolysis

Identifiers

PMID42794846

DOI10.3390/ijms27188421

PMCPMC13607522

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.