Evidence map›Paper›PMID 42794104›Full record

ArticleFoods (Basel, Switzerland)2026

Terroir Rediscovered: A Polyphasic Approach to Autochthonous Lactic Acid Bacteria as Functional and Microbial Heritage Resources in Portuguese PDO Cheeses.

Susana Serrano, Joana Monteiro Marques, Cinthia Alves-Barroco, Susana Morais, Catarina Araújo, Jéssica Elias, Maria Teresa Barreto-Crespo, Teresa Semedo-Lemsaddek

Abstract read
In one paragraph

Article in Foods (Basel, Switzerland), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Susana SerranoCIISA-Center for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.ORCID 0009-0001-6715-5643
Joana Monteiro MarquesCIISA-Center for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.ORCID 0000-0001-8944-3235
Cinthia Alves-BarrocoUCIBIO-Applied Molecular Biosciences Unit, Department of Life Sciences, NOVA School of Science and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.ORCID 0000-0002-3526-0780
Susana MoraisLaboratory of Molecular Microbiology of Human Pathogens, ITQB NOVA-Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, 2780-157 Oeiras, Portugal.ORCID 0009-0007-5741-3956
Catarina AraújoCIISA-Center for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.ORCID 0009-0009-2061-0299
Jéssica EliasCIISA-Center for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.
Maria Teresa Barreto-CrespoiBET-Instituto de Biologia Experimental e Tecnológica, Av. República, Qta. do Marquês, 2780-157 Oeiras, Portugal.ORCID 0000-0002-5605-1419
Teresa Semedo-LemsaddekCIISA-Center for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, 1300-477 Lisbon, Portugal.ORCID 0000-0002-8349-3802

Funding

Fundação para a Ciência e Tecnologia LA/P/0059/2020-AL4ANIMALSFundação para a Ciência e Tecnologia LA/P/0087/2020Fundação para a Ciência e Tecnologia PTDC/OCEETA/1785/2020Fundação para a Ciência e Tecnologia UI/BD/153073/2022Fundação para a Ciência e Tecnologia UID/276/2025Fundação para a Ciência e Tecnologia UIDB/04462/2020
6 · The paper itself

Abstract

Portuguese Protected Designation of Origin (PDO) cheeses from Azeitão and Nisa harbor unique autochthonous microbial communities integral to their terroir, sensory identity, and authenticity. Rediscovering and preserving this microbial heritage is essential for safeguarding traditional cheesemaking practices and unlocking the biotechnological potential of indigenous microorganisms. This study used a polyphasic approach to isolate, preserve, and functionally characterize lactic acid bacteria (LAB) from these cheeses, aiming both to conserve autochthonous microbial resources and evaluate their suitability for food applications and preliminary probiotic-related traits. From 2304 bacterial isolates, 119 representative LAB were selected and screened for safety based on hemolytic and gelatinolytic activities and antimicrobial susceptibility. Technological potential was assessed through acidification, proteolytic and lipolytic activities, and antagonism against foodborne pathogens, while probiotic traits were evaluated via tolerance to acidic pH and bile salts, survival under simulated gastrointestinal conditions, and auto- and co-aggregation. Thirty-nine isolates exhibited desirable technological traits, including proteolytic and lipolytic activities and broad-spectrum pathogen antagonism. Among these, three isolates (two

Indexed as

autochthonous lactic acid bacteriafood safetymicrobial heritagepolyphasic characterizationPortuguese traditional cheesesprobiotic potentialstarter and adjunct culturestechnological properties

Identifiers

PMID42794104
PMCPMC13606075

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.