Selection and Validation of qRT-PCR Reference Genes in

Miaomiao Liu, Xiangyu Lin, Xiumei Zhou

Abstract read

In one paragraph

Article in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Miaomiao LiuHenan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plant, School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China.ORCID 0000-0003-3934-9448

Xiangyu LinHenan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plant, School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China.

Xiumei ZhouHenan Engineering Research Center of the Development and Utilization of Characteristic Horticultural Plant, School of Horticulture and Landscape Architecture, Henan Institute of Science and Technology, Xinxiang 453003, China.

Funding

Henan Provincial Science and Technology Research Project 232102111081

6 · The paper itself

Abstract

Selecting and validating appropriate reference genes is a prerequisite for accurate gene expression analysis by means of quantitative real-time PCR (qRT-PCR), particularly in plant species with limited molecular research.

Indexed as

BestKeeperComparative ΔCtElsholtzia stauntoniigene expression normalizationgeNormNormFinderreference gene stabilityRefFinder

Identifiers

PMID42793289

DOI10.3390/cimb48090933

PMCPMC13605697

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.