Evidence map›Paper›PMID 42793264›Full record

ArticleCurrent issues in molecular biology2026

Rapid Generation of High-Affinity Rabbit Anti-Mouse IgG Monoclonal Antibodies by High-Throughput Single-B-Cell Sorting and Recombinant Expression.

Ying Fu, Fang Li, Hengping Wang, Xueyuan Wang, Huiyan Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current issues in molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ying FuJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132013, China.
Fang LiJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132013, China.
Hengping WangJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132013, China.
Xueyuan WangJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132013, China.
Huiyan WangJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132013, China.

Funding

Jilin Province Science and Technology Department YDZJ202402030CXJD
6 · The paper itself

Abstract

Rabbit monoclonal antibodies (RabMAbs) are valuable for biomedical research and diagnostic applications because of their high affinity, specificity, and broad epitope recognition; here, we established an integrated phenotype-linked workflow for rapid RabMAb discovery using serum-derived polyclonal mouse IgG as a proof-of-concept model antigen. Three Big-Eared White rabbits were immunized in parallel, and the rabbit exhibiting the highest serum endpoint titer was selected for the complete downstream single-B-cell discovery workflow. Approximately 5 × 10

Indexed as

biolayer interferometryExpiCHO expressionhigh-throughput single-B-cell sortingmicrofluidic encapsulationnative VH/VL pairingrabbit monoclonal antibodiesV(D)J sequencing

Identifiers

PMID42793264
PMCPMC13605436

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.