Evidence map›Paper›PMID 42792535›Full record

ArticleBiology2026

Integrated Transcriptomic and Metabolomic Analysis Reveals Key Genes in Fatty Acid and Flavonoid Biosynthesis in Macadamia Nut (

Qiujin Tan, Xiaokang Fan, Chunheng Zhou, Xiyun Huang, Zhenzhen Pan, Yuanrong Wei, Xiaozhou Yang, Wenlin Wang

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Article in Biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

8 authors.

Qiujin TanGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.
Xiaokang FanCollege of Tropical Agriculture and Forestry, Hainan University, Danzhou 571737, China.
Chunheng ZhouGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.
Xiyun HuangGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.
Zhenzhen PanGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.
Yuanrong WeiGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.
Xiaozhou YangGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.
Wenlin WangGuangxi Key Laboratory of Smart Agriculture for Characteristic Horticultural Crops, Chongzuo Field Integrated Scientific Observation and Research Station of the Ministry of Agriculture and Rural Affairs, Guangxi South Subtropical Agricultural Science Research Institute, Longzhou 532415, China.

Funding

Guangxi Academy of Agricultural Science 2026YT023Guangxi Natural Science Foundation 2025GXNSFAA069142Stable Funding Program for Scientific Research Team Project NYS2025ST01
6 · The paper itself

Abstract

bHLH transcription factors are central to plant development and to the metabolism of fatty acids and flavonoids. Through the transcriptomic, metabolomic, and phytohormone/elemental profiling of two macadamia varieties with divergent fatty acid content, 'Guire No. 1' (GR1) and 'A4', we identified 142 MibHLH transcription factors, classified into 21 subfamilies, of which 14 show collinearity with Arabidopsis AtbHLH members. MibHLH46 was markedly differentially expressed, and its expression strongly correlated with that of fatty acid biosynthesis genes. Promoter analysis further revealed abundant MYC and G-box elements in fatty acid and flavonoid biosynthetic genes. Comparative metabolomics showed that 'GR1' accumulates higher levels of several mineral elements (Fe, Ca, Mg) and abscisic acid (ABA) than 'A4'. These transcriptomic trends were confirmed by qRT-PCR, which validated the up-regulation of genes related to fatty acid oxidation, α-linolenic acid metabolism, and the flavonoid pathway in 'GR1'. We propose that MibHLH46, as a candidate regulator, contributes to kernel fatty acid and flavonoid metabolism by transcriptionally regulating key biosynthetic genes, providing a basis for identifying regulatory genes in macadamia lipid metabolism.

Indexed as

fatty acidflavonoidmacadamia nutMibHLH46transcriptome

Identifiers

PMID42792535
PMCPMC13603688

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