ArticleAnimals : an open access journal from MDPI2026
Integrated Bulk and Single-Nucleus Transcriptomics Characterizes the Developmental and Regional Specialization of Chicken Abdominal Fat-Associated Tissue.
Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Excessive abdominal fat deposition reduces feed efficiency and edible carcass yield in chickens, yet its developmental and regional specialization remains poorly understood. We generated 93 bulk RNA sequencing (RNA-seq) libraries from 15 fat-associated anatomical regions at embryonic day 16 (E16) and 16 regions at 2 weeks post-hatch (2w) in six Xinghua chickens. Exploratory single-nucleus RNA sequencing was additionally performed on abdominal region (AR) tissue from one individual at each stage. Repeated-measures-aware analysis identified developmental stage as the major source of transcriptomic variation, with 4556 genes expressed at higher levels at E16 and 4528 at 2w. E16-associated programs were enriched for proliferation, morphogenesis, and structural development, whereas postnatal programs were dominated by immune and stimulus-response processes. Stage-specific weighted gene co-expression network analysis (WGCNA) identified substantial regional heterogeneity. AR showed stage-dependent signatures, including higher developmental and morphogenetic programs and lower lipid-metabolic programs at E16, and higher cell-cycle-related and lower developmental and fatty-acid-metabolic programs at 2w. Single-nucleus analysis identified adipose-lineage and multiple non-adipocyte populations, and cross-omics mapping showed that AR-associated signatures were distributed across multiple cellular compartments. These results suggest stage-dependent, multicellular transcriptional specialization of chicken AR tissue.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.