ArticleAnimals : an open access journal from MDPI2026
Dietary Rumen-Protected Gamma-Aminobutyric Acid Supplementation to Alleviate Stress in Beef Cattle Caused by Long-Distance Transport by Improving Antioxidant Capacity, Immune Function, and Hormonal Balance: A Related Study.
Article in Animals : an open access journal from MDPI, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
γ-aminobutyric acid (GABA), an inhibitory neurotransmitter, plays an important role in regulating stress responses through modulation of the hypothalamic-pituitary-adrenal axis, neuroendocrine activity, antioxidant defense, and immune function. Therefore, this study evaluated the effects of dietary rumen-protected γ-aminobutyric acid (RP-GABA) supplementation on alleviating long-distance transport-induced stress in beef cattle. Forty-eight healthy Simmental bulls were randomly assigned to four treatment groups: CON, G-L, G-M, and G-H, receiving RP-GABA at 0, 300, 500, and 700 mg/kg of concentrate dry matter, respectively. The experiment lasted 53 days, including a 21-day pre-transport feeding period, a 2-day transportation period, and a 30-day post-transport feeding period. Samples were collected on experimental Days 21, 23, and 54. The results showed that long-distance transport impaired antioxidant status, immune function, and stress hormone secretion in beef cattle. The responses to different dietary RP-GABA supplementation levels varied among serum parameters. Long-distance transport reduced triiodothyronine and thyroxine (T4) concentrations, while the G-L group alleviated these reductions and maintained higher levels than the CON group after transport (Day 23). The G-M group enhanced glutathione peroxidase (GSH-Px) activity after transport (Day 23) (
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