Evidence map›Paper›PMID 42790701›Full record

ArticleMolecular metabolism2026

Biosensor cell array reveals temporal GABA secretion dynamics from pancreatic islets.

Austin E Stis, Charles S Lazimi, Sandra M Ferreira, Alexandra E Cuaycal, Dylan Smurlick, D Walker Hagan, Taylor Nakayama, Sunil P Gandhi, Emery Smith, Timothy P Spicer and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular metabolism, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Austin E StisJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Charles S LazimiJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Sandra M FerreiraJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Alexandra E CuaycalJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA; Department of Infectious Diseases and Immunology, University of Florida, Gainesville, FL, USA.
Dylan SmurlickJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
D Walker HaganJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Taylor NakayamaDepartment of Neurobiology & Behavior, University of California, Irvine, Irvine, CA, USA.
Sunil P GandhiDepartment of Neurobiology & Behavior, University of California, Irvine, Irvine, CA, USA.
Emery SmithThe Herbert Wertheim UF Scripps Institute Molecular Screening Center, Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation and Technology, Jupiter, FL, USA.
Timothy P SpicerThe Herbert Wertheim UF Scripps Institute Molecular Screening Center, Department of Molecular Medicine, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation and Technology, Jupiter, FL, USA.
Edward A PhelpsJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA; Department of Pathology, Immunology and Laboratory Medicine, University of Florida, Gainesville, FL, USA; University of Florida Diabetes Institute, Gainesville, FL, USA. Electronic address: ephelps@bme.ufl.edu.

Funding

Project 3P01AI042288 · NIAID · UNIVERSITY OF FLORIDA · PI Todd Michael Brusko · 1997 to 2026
$32.9M
The Human Islet Distribution Coordinating Center (UC4)UC4DK098085 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI EVANS-MOLINA, CARMELLA, NILAND, JOYCE CAROL · 2012 to 2017
$25.6M
Engineering a Human Microphysiological System for the Characterization of Islet-Immune InteractionsUH3DK122638 · NIDDK · UNIVERSITY OF FLORIDA · PI AGARWAL, ASHUTOSH, BRUSKO, TODD MICHAEL · 2021 to 2023
$3.0M
Local immune modulation for beta cell replacement therapy in type 1 diabetesR01DK132387 · NIDDK · UNIVERSITY OF FLORIDA · PI PHELPS, EDWARD, RUSS, HOLGER A. · 2022 to 2025
$2.1M
Interdisciplinary Graduate Program in Type 1 Diabetes and Biomedical EngineeringT32DK108736 · NIDDK · UNIVERSITY OF FLORIDA · PI MARK A. ATKINSON, Benjamin George Keselowsky · 2017 to 2026
$1.9M
Mechanism and dynamics of islet GABA signalingR01DK124267 · NIDDK · UNIVERSITY OF FLORIDA · PI PHELPS, EDWARD · 2021 to 2025
$1.8M
Regional and lobular heterogeneity of human pancreas morphology and function in type 1 diabetes pathogenesisR01DK123292 · NIDDK · UNIVERSITY OF FLORIDA · PI ATKINSON, MARK A., SPEIER, STEPHAN · 2020 to 2023
$1.7M
NIAID NIH HHS P01 AI042288NIDDK NIH HHS R01 DK123292NIDDK NIH HHS R01 DK124267NIDDK NIH HHS R01 DK132387NIDDK NIH HHS T32 DK108736NIDDK NIH HHS UC4 DK098085NIDDK NIH HHS UH3 DK122638
6 · The paper itself

Abstract

Pancreatic beta cells have the unique function of synthesizing and secreting high amounts of the inhibitory neurotransmitter γ-aminobutyric acid (GABA). The mechanism of GABA secretion, whether vesicular or channel-mediated, is debated. Our study reveals surprising temporal complexity in the pattern of islet GABA secretion. We used insulin secretion modulators to demonstrate that GABA release is not directly correlated with insulin secretion. VGAT reporter mice also showed that beta cells do not express the requisite vesicular GABA transporter (VGAT) for vesicular GABA release. Instead, GABA is secreted from the cytosol in pulses that depend on expression of the LRRC8D subunit of the volume regulatory anion channel (VRAC). We further demonstrate the dynamic coordination of GABA release with calcium influx in beta cells and dependence on beta cell depolarization. These results suggest a model where GABA is released during the peaks of beta cell calcium oscillations to provide feedback which strengthens and reinforces the oscillation waveform.

Indexed as

Biosensor CellsCalcium ImagingDiabetesGABALRRC8A/D

Identifiers

PMID42790701
PMCPMC13677039

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.