ArticleCurrent protocols2026
An Integrated Platform for the Comprehensive Analysis of Host-Fungal Interactions in the Murine Tongue.
Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- An Integrated Platform for the Comprehensive Analysis of Host-Fungal Interactions in the Murine Tongue.Current protocols · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
This protocol describes a mouse model of Candida albicans oral colonization that establishes stable, long-term fungal persistence in the oral cavity, thereby recapitulating key features of human commensal colonization, including the induction of sustained adaptive antifungal immunity. The model enables the analysis of host-fungal interactions across multiple experimental readouts. Procedures are provided for isolation of single-cell suspensions from the whole tongue, the tongue epithelium, and the draining lymph nodes for flow cytometric analysis, supporting both enumeration and extended functional characterization of immune cell populations. Antigen-specific T cell responses can be assessed by re-stimulation of isolated T cells with C. albicans-pulsed dendritic cells prior to staining and analysis. Additional methods include quantification of fungal burden in tongue tissue, histological assessment of fungal localization and immune cell infiltration, and isolation of high-quality RNA using an optimized extraction workflow compatible with RT-qPCR and RNA sequencing. Collectively, these protocols provide a flexible and integrated platform for studying immune responses and host-fungal interactions during persistent oral colonization. © 2026 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Oral colonization of mice with Candida albicans Basic Protocol 2: Preparation of single-cell suspensions from the murine whole tongue, tongue epithelium, and draining lymph nodes Basic Protocol 3: Re-stimulation of cLNs and tongue T cells for qualitative and quantitative assessment of T cell effector functions Basic Protocol 4: Flow-cytometry-based analysis of tongue immune cell populations Support Protocol 1: Evaluation of fungal burden in the tongue of C. albicans-colonized mice Support Protocol 2: Visualization of tissue integrity, tissue inflammation, and fungal localization by histology Support Protocol 3: Extraction of total RNA from the tongue of C. albicans-colonized mice for RNA sequencing and RT-qPCR.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.