ArticleCurrent protocols2026
PDBe PISA: Enhanced Analysis of Macromolecular Interactions.
Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- PDBe PISA: Enhanced Analysis of Macromolecular Interactions.Current protocols · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Protein interactions play a pivotal role in determining the biological functions of living cells. Gaining structural insights into protein interfaces can illuminate their role in function and diseases and highlight their potential as therapeutic targets. In response, there has been a marked increase in efforts by the scientific community to decipher the nuances of macromolecular interfaces, aiming to predict interactions and binding specificity of different components within complexes. The "Proteins, Interfaces, Structures, and Assemblies" (PISA) software has become a standard tool for analyzing intermolecular interactions and elucidating macromolecular assemblies from molecular structures. This article presents detailed protocols for using Protein Data Bank in Europe (PDBe) PISA API, a new Application Programming Interface (API) for enhanced analysis of protein assembly interfaces, using an updated version of PISA software. Compared to its predecessor, the updated PISA software expands interface data, increasing the coverage and detail of interaction information for assemblies and introduces greater computational flexibility, allowing users to perform targeted interface analyses for selected assemblies efficiently. The PDBe team uses the PISA API to provide interaction data in a JavaScript Object Notation (JSON) format for weekly Protein Data Bank (PDB) releases to support the efforts of the broader scientific community. Our protocols are a comprehensive guide to harnessing macromolecular interaction data using PISA. We delve into practical examples, from querying interaction and interface data from JSON files to extracting interaction data for assemblies cataloged in the PDB archive using programmatic access and bulk download via File Transfer Protocol (FTP). © 2026 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Programmatic access to interaction data with PDBe PISA API Basic Protocol 2: FTP access to interaction data of assemblies Basic Protocol 3: Analysis of assembly interfaces and interactions.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.