Observational studyRevista da Sociedade Brasileira de Medicina Tropical2026
Accelerating Proficiency in Minimally Invasive Autopsy for Infectious Disease Diagnosis and Research: A Strategic Framework.
Observational study in Revista da Sociedade Brasileira de Medicina Tropical, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundConventional autopsy is the gold standard for postmortem examination, but its use is constrained by biosafety, infrastructure, and acceptability concerns. Ultrasound-guided minimally invasive autopsy and ultrasound-guided minimally invasive tissue sampling (US-MITS) offer practical alternatives for tissue sampling in infectious diseases. The aim of this study was to present a standardized protocol for multiorgan US-MITS and evaluate its performance in terms of success rates, accuracy, and sample quality.
methodsAn observational study was conducted on 13 decedents with reverse transcription-polymerase chain reaction (RT-PCR)-confirmed coronavirus disease 2019 in Salvador, Brazil. Using a portable ultrasound machine and 14G semi-automatic Tru-Cut needles, samples were collected from the skin, liver, spleen, kidneys, pancreas, lungs (four-quadrant bilateral sampling), heart, brain, and skeletal muscle. The workflow included routine histological examinations (hematoxylin and eosin, and special stains), electron microscopy, and molecular testing via Ion Torrent sequencing for severe acute respiratory syndrome coronavirus 2 variant calling. Performance was evaluated by measuring the success rates and accuracy (ratio of target tissue to contaminants) using high-resolution slide scanning and digital morphometry.
resultsSampling success ranged from 77% to 100% across the heart, lungs, liver, spleen, and kidneys. Four-quadrant lung sampling yielded comprehensive histological representation. Contamination was generally low, except in the pancreas and lower medial lung quadrant. Tissue suitable for electron microscopy was obtained from most organs; severe acute respiratory syndrome coronavirus 2 was detected in all 13 lungs, and variants were assigned in 10 cases.
conclusionWith minimal training, teams can perform US-MITS. Using a traceable, standardized workflow accelerates proficiency while enabling diagnostic-grade samples to be produced for histological examinations, ultrastructural analysis, and molecular testing in resource-limited and outbreak settings.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.