ArticleBurns & trauma2026
Innovative research on predicting skin flaps necrosis through quantification of the USP4/TNFAIP2 regulated 'M1 macrophages-Tregs axis' via skin barrier monitoring technology.
Article in Burns & trauma, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Current methods used for assessing random-pattern skin flaps (RPSFs) viability are invasive and subjective. Therefore, this study aimed to establish an accurate and non-invasive method for predicting RPSFs prognosis using skin barrier monitoring technology. Methods: Gene ontology (GO) analysis was employed to identify the potential pathways associated with RPSFs necrosis. Skin barrier monitoring technology, immunohistochemistry (IHC), immunofluorescence (IF), and western blotting (WB) were employed to assess skin barrier integrity, quantify ferroptosis level, and evaluate the recruitment of M1 macrophages and regulatory T cells (Tregs). Clinical samples were also included to validate the accuracy and efficacy of skin barrier monitoring technology in predicting RPSFs necrosis. Results: The necrotic process in RPSFs was characterized by increased infiltration of M1 macrophages and Tregs, accompanied by activated ferroptosis and dysfunction of skin barrier. Notably, Tregs infiltration significantly reduced the necrotic area and restored skin barrier integrity of RPSFs by inhibiting ferroptosis and polarization of M1 macrophages. Mechanistically, USP4 regulated the interaction of M1 macrophages and Tregs (M1 macrophages-Tregs axis) via stabilization of TNFAIP2. Analysis of clinical samples revealed that skin barrier monitoring technology could serve as predictive strategy for RPSFs necrosis. Conclusions: This study demonstrated that the USP4/TNFAIP2 pathway would regulate skin barrier function and ferroptosis via 'M1 macrophages-Tregs axis' in RPSFs. And the skin barrier monitoring technology could serve as reliable method for RPSFs necrosis prediction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.