Hijacking host RNA polymerase for processive in situ mutagenesis.

Yuou Sheng, Huizhen Ni, Shihao Yang, Wenliang Hao, Chong Zhang

Abstract read

In one paragraph

Article in Synthetic and systems biotechnology, 2027. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yuou ShengMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

Huizhen NiMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

Shihao YangSchool of Life Sciences, Tsinghua University, Beijing, 100084, China.

Wenliang HaoMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

Chong ZhangMOE Key Laboratory for Industrial Biocatalysis, Institute of Biochemical Engineering, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Targeted mutagenesis is a crucial tool for in vivo continuous evolution. However, existing methods for long-range targeted mutagenesis in vivo often face limitations such as low efficiency, poor host compatibility, operational complexity, and uneven distribution of mutations across these windows. In this study, we developed a novel in vivo Targeted Assisted Mutagenesis via Endogenous RNAP tool (TAMER) by functionally fusing the endogenous ω subunit of RNAP with a deaminase and dCas9. This tool achieved a mutagenesis efficiency of 1.28 × 10

Indexed as

Directed evolutionIn situ evolutionMicrobial chassisTargeted mutagenesisTranscription-coupled mutagenesis

Identifiers

PMID42787729

DOI10.1016/j.synbio.2026.09.002

PMCPMC13602049

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.