ArticleFrontiers in chemistry2026
Rapid subtype-specific detection of respiratory syncytial virus A and B using an RNase HII-dependent RT-LAMP lateral flow assay.
Article in Frontiers in chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Introduction: Rapid and simultaneous detection of respiratory syncytial virus A (RSV A) and RSV B with subtype discrimination is important for point-of-care (POC) diagnostics and epidemiological surveillance. Methods: We developed the RSV A/B rh-RT-LAMP-NALF assay, a duplex reverse transcription loop-mediated isothermal amplification (RT-LAMP) system combined with nucleic acid lateral flow (NALF) detection for simultaneous subtype-specific detection of RSV A and RSV B from nasopharyngeal swab specimens. To improve specificity during multiplex RT-LAMP, blocked inner primers, activated by RNase HII, were incorporated to suppress non-specific amplification. Subtype-specific detection was achieved on a single PCRD multiplex strip using 5'-FAM-and 5'-DIG-labeled loop primers for RSV A (T1 line) and RSV B (T2 line), respectively. Results: The analytical limit of detection was 4 × 10 Discussion: Overall, the RSV A/B rh-RT-LAMP-NALF assay provides a rapid, accurate, and subtype-specific POC diagnostic platform for RSV detection and surveillance.
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