Evidence map›Paper›PMID 42787216›Full record

ArticleFrontiers in cellular and infection microbiology2026

Impact of clinical sample processing time on the performance and T-cell response of the T-SPOT.TB assay.

Yue Chang, Guiren Ruan, Yaxu Liu, Baotong Zhou, Lifan Zhang, Yang Han

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In one paragraph

Article in Frontiers in cellular and infection microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yue Chang *Department of Infectious Diseases, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Guiren Ruan *Department of Infectious Diseases, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Yaxu LiuDepartment of Health Care, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Beijing, China.
Baotong ZhouDepartment of Infectious Diseases, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Lifan ZhangDepartment of Infectious Diseases, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Yang HanDepartment of Infectious Diseases, State Key Laboratory of Complex Severe and Rare Disease, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objectives: T-SPOT. Methods: Outpatients undergoing T-SPOT. Results: Among 443 patients, 282 underwent 24-h delayed testing. Agreement with standard-timing results was 93.3% without TCX (κ=0.859) and 93.0% with TCX (κ=0.860), comparable to fresh-sample reproducibility (95.0%; κ=0.840; all p<0.001). Multivariable analysis identified initial borderline results (OR = 15.21, p<0.001) and 24-h delay (p<0.05) as independent predictors of T-SPOT.TB reversion, regardless of TCX use. Antigen-well spot-forming cell (SFC) counts were significantly reduced after 24-h delay (p<0.001); TCX did not mitigate this decline (p=1.000). Positive-control SFC counts remained stable (p=0.602). Conclusions: A 24-h processing delay demonstrated high concordance with standard-timing T-SPOT.

Indexed as

Interferon-gamma Release TestsMycobacterium tuberculosisSpecimen HandlingT-LymphocytesTuberculosisAdultFemaleHumansInterferon-gammaMaleMiddle AgedProspective StudiesReproducibility of ResultsSensitivity and SpecificityTime FactorsInterferon-gammadiagnosisinterferon-gamma release assayMycobacterium tuberculosis infectionT-Cell XtendT-SPOT.TB

Identifiers

PMID42787216
PMCPMC13600888

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.