Evidence map›Paper›PMID 42785309›Full record

ArticleStem cell reports2026

Directing GABAergic neuronal identity from human iPSCs through developmental patterning.

Ruiqi Hu, Linda L Boshans, Yiran Tao, Bohan Zhu, Junlong Li, Xindi Li, Rui Dang, Marianna Liang, Peiwen Cai, Mark Youssef and 9 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors.

Ruiqi HuNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Linda L BoshansNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Yiran TaoNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Bohan ZhuDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Junlong LiCenter for NeuroMetabolism, The Child Health Institute of New Jersey, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA; Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA.
Xindi LiCenter for NeuroMetabolism, The Child Health Institute of New Jersey, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA; Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA.
Rui DangDepartment of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
Marianna LiangNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Neurology, Icahn School of Medicine at Mount Sinai, 1425 Madison Avenue, New York, NY 10029, USA.
Peiwen CaiDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Mark YoussefNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Gizem InakNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Yingnan SongDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Xuran WangDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Alexander M TsankovDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Joseph D BuxbaumDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Seaver Autism Center for Research and Treatment, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Psychiatry, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.
Peng JiangDepartment of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
Zhiping P PangCenter for NeuroMetabolism, The Child Health Institute of New Jersey, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA; Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, New Brunswick, NJ 08901, USA.
Sai MaDepartment of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address: sai.ma@mssm.edu.
Nan YangNash Family Department of Neuroscience, Friedman Brain Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Alper Center for Neurodevelopment and Regeneration, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Institute for Regenerative Medicine, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address: nan.yang1@mssm.edu.

Funding

Conduits: Mount Sinai Health System Translational Science HubUL1TR004419 · NCATS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Rosalind J Wright · 2022 to 2026
$46.4M
Deciphering the neural basis of alcohol use disorders using human and mouse neuronsR01AA023797 · NIAAA · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI ZHIPING P. PANG · 2016 to 2026
$4.8M
Functional mapping of noncoding regulatory variants in human neuronal subtypes3.R01MH129372 · NIMH · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Nan Yang · 2022 to 2026
$3.4M
Loss-of-Function Analyses of SETD1A in Human Neural ModelsR01MH125528 · NIMH · RUTGERS BIOMEDICAL AND HEALTH SCIENCES · PI MING, GUO-LI, PANG, ZHIPING P. · 2021 to 2025
$3.2M
COVID and Translational Science supercomputer (CATS)S10OD030463 · OD · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KOVATCH, PATRICIA · 2021 to 2021
$2.0M
Big Omics Data Engine 2 SupercomputerS10OD026880 · OD · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI KOVATCH, PATRICIA · 2019 to 2019
$2.0M
Cellular and Molecular Mechanisms of Brain Dysfunction in NF1R01NS116057 · NINDS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI YANG, NAN · 2020 to 2024
$2.0M
Generation and characterization of MGE-derived GABAergic neurons from human pluripotent stem cellsR21MH119580 · NIMH · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI YANG, NAN · 2019 to 2020
$466k
Pooled Optical Imaging, Neurite Tracing, and Morphometry Across Perturbations (POINT-MAP).R21MH131600 · NIMH · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI BUXBAUM, JOSEPH D., YANG, NAN · 2023 to 2023
$465k
Profiling of Midbrain Neuronal Vulnerability in Parkinson's DiseaseF31NS137751 · NINDS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI Marianna Liang · 2025 to 2026
$99k
NCATS NIH HHS UL1 TR004419NIAAA NIH HHS R01 AA023797NIH HHS S10 OD026880NIH HHS S10 OD030463NIMH NIH HHS R01 MH125528NIMH NIH HHS R01 MH129372NIMH NIH HHS R21 MH119580NIMH NIH HHS R21 MH131600NINDS NIH HHS F31 NS137751NINDS NIH HHS R01 NS116057
6 · The paper itself

Abstract

GABAergic interneurons are implicated in numerous neurodevelopmental and neuropsychiatric disorders. Transcription factor (TF)-mediated induction of human pluripotent stem cells (PSCs) rapidly generates GABAergic neurons, yet their regional identity remains poorly defined. Here, we show that the chromatin state of the starting population constrains the neuronal identities specified by ASCL1 and DLX2. Brief neural patterning via dual-SMAD and WNT inhibition prior to TF induction biases PSCs toward GABAergic neurons with forebrain-associated identities, whereas TF induction of PSCs yields neurons with broader regional signatures, including hypothalamic and thalamic programs. Benchmarking multiple approaches against a human fetal brain atlas reveals distinct regional identity biases and disease gene enrichment profiles across methods, providing a framework for selecting differentiation strategies for disease modeling. Applying this approach to ADNP syndrome, we demonstrate that the recurrent p.Tyr719

Indexed as

Body PatterningGABAergic NeuronsInduced Pluripotent Stem CellsBasic Helix-Loop-Helix ProteinsCell DifferentiationGene Expression Regulation, DevelopmentalHomeodomain ProteinsHumansInterneuronsNeurodevelopmentTranscription FactorsASCL1 protein, humanBasic Helix-Loop-Helix ProteinsDistal-less homeobox proteinsHomeodomain ProteinsTranscription Factorsdifferentiationdisease modelingGABAergic neuronshuman stem cells

Identifiers

PMID42785309
PMCPMC13681393

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.