Isolation, identification and phylogenetic analysis of a novel goose astrovirus 2 strain.

Yingjie Gu, Xuming Hu, Huangjun Shen, Huixian Wu, Guangzhong Peng, Suyu Fan, Xiao Wang, Qi Xu

Abstract read

In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yingjie GuJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Xuming HuJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Huangjun ShenJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Huixian WuJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Guangzhong PengJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Suyu FanJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Xiao WangJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.

Qi XuJiangsu Key Laboratory for Animal Genetic, Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; Institute of Epigenetics and Epigenomics, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address: xuqi@yzu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An outbreak of acute nephritis and growth retardation occurred on a goose farm in Jiangsu Province, China, in 2025. Affected goslings exhibited typical symptoms of gout, and RT-PCR confirmed infection with goose astrovirus 2 (GAstV-2) alone. A novel strain, designated JSYC2503, was isolated from liver tissue using Leghorn male hepatoma (LMH) cells. This strain induced clear cytopathic effects within 72 h and remained stable after 20 serial blind passages. The complete genome of JSYC2503 consisted of 7,180 nucleotides and displayed the typical astrovirus genome organization, including a conserved ribosomal frameshift signal and a stem-loop II-like motif (s2m) in the 3'-UTR. Phylogenetic analysis placed JSYC2503 within the GAstV-2 clade, showing the highest sequence identity to the Chinese GAstV-2 strain JXNC1 (99.6% at the whole-genome level) but much lower identity (29.9%-47.1%) to other avian astroviruses. In an experimental infection study, 3-day-old goslings inoculated intraperitoneally with JSYC2503 developed clinical signs, showed 50% mortality by day 6, and exhibited pathological changes including splenomegaly, bursal atrophy, and urate deposition in the kidneys. Viral shedding was continuously detected in cloacal swabs. These results indicate that JSYC2503 is a highly pathogenic GAstV-2 strain that may pose a substantial threat to goose health, and its genomic characterization provides insight into the evolution and pathogenesis of goose astroviruses.

Indexed as

Goose astrovirus 2PathogenicityVirus identificationVirus isolation

Identifiers

PMID42785077

DOI10.1016/j.psj.2026.107813

PMCPMC13634697

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