ArticlePoultry science2026
Quercetin exhibits antiviral activity against duck Tembusu virus in vitro and in vivo.
Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Duck Tembusu virus (DTMUV) is an avian pathogenic flavivirus that causes substantial economic losses to the poultry industry in China and Southeast Asia. The current antiviral drugs for the prevention and treatment of DTMUV infection are limited. Quercetin is a natural flavonoid widely found in fruits and vegetables and has been reported to exhibit antiviral activity against many viruses. However, whether quercetin possesses antiviral activity against DTMUV remains unknown. Therefore, the present study aimed to investigate the antiviral efficacy of quercetin against DTMUV. A Cell Counting Kit-8 (CCK-8) assay was performed to evaluate the cytotoxicity of quercetin in BHK-21 cells. The antiviral activity of quercetin was assessed using 50% tissue culture infectious dose (TCID₅₀) assay, real-time quantitative PCR (RT-qPCR), Western blotting and indirect immunofluorescence assay (IFA). Molecular docking analysis was conducted to predict potential viral targets. Moreover, the antiviral efficacy of quercetin in vivo was further evaluated in DTMUV-infected goslings. The results of the CCK-8 assay revealed that quercetin at concentrations up to 40 μM had no significant effect on BHK-21 cells. Quercetin significantly inhibited DTMUV replication in BHK-21 cells in a dose- and time-dependent manner, as demonstrated by reduced viral titres, RNA copy numbers, and E protein expression. Direct virucidal assays revealed that quercetin could directly inactivate DTMUV particles. Adsorption and post-adsorption assays revealed that quercetin inhibited DTMUV infection during the post-adsorption stage. Furthermore, molecular docking predictions indicate that the viral NS5 protein is a potential target for quercetin. In vivo, quercetin treatment significantly improved the survival rate of infected goslings; reduced viral loads in the liver, spleen, and brain; and alleviated gross pathological lesions and histopathological damage caused by DTMUV infection. In conclusion, quercetin exhibits potent anti-DTMUV activity both in vitro and in vivo. These findings provide a theoretical basis for the development of quercetin as a natural antiviral agent and highlight its potential application in the prevention and control of DTMUV infection.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.