ArticlePLoS pathogens2026
GM000287 is a conserved determinant of serum resistance and virulence in Klebsiella pneumoniae.
Article in PLoS pathogens, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Klebsiella pneumoniae (Kp) is an opportunistic Gram-negative pathogen responsible for severe community- and healthcare-associated infections. Resistance to complement-mediated killing is an important determinant of Kp survival in human serum, yet mechanisms beyond capsular polysaccharide-mediated protection remain incompletely understood. Here, using comparative proteomic approaches, we identified a previously uncharacterized protein, GM000287, as a conserved determinant of serum resistance and virulence in Kp. Human serum exposure induced expression of the bacterial type II secretion system (T2SS), and deletion of the essential T2SS components GspD/GspE increased susceptibility to human serum killing. Importantly, disruption of GspD/GspE attenuated virulence in a murine pneumonia model. Comparative secretome analysis identified GM000287 as a T2SS-associated envelope protein that contributes to serum survival. Loss of GM000287 increased C3b production and factor B deposition on the bacterial surface during human serum exposure, consistent with enhanced alternative pathway amplification. In a murine pneumonia model, deletion of GM000287 reduced bacterial persistence and dissemination and attenuated virulence. Given the minimal bactericidal activity of mouse serum against Kp, indicating an additional role for GM000287 during infection. Importantly, GM000287 was highly conserved among Klebsiella species and broadly distributed among members of the Enterobacteriaceae family, and its contribution to serum resistance was validated in genetically diverse Kp clinical isolates. Together, these findings identify GM000287 as a conserved determinant of human serum resistance and in vivo virulence and reveal distinct contributions of GM000287 to K. pneumoniae fitness across different host-associated environments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.